Oblongifolin C (OC), a natural small molecule compound extracted from Garcinia yunnanensis Hu, has been previously shown to have anti-cancer effect, but the anti-allergic effect of OC has not yet been investigated. The aim of the present study is to determine the anti-allergic effect of OC on IgE/Ag-induced mouse bone marrow-derived mast cells (BMMCs) and on the passive systemic anaphylaxis (PSA) reaction in mice. OC clearly suppressed cyclooxygenase-2 (COX-2)-dependent prostaglandin D-2 (PGD(2)) generation as well as leukotriene C-4 (LTC4) generation and the degranulation reaction in IgE/Ag-stimulated BMMCs. Biochemical analyses of the IgE/Ag-mediated signaling pathways showed that OC suppressed the phosphorylation of phospholipase C gamma 1 (PLC gamma 1)-mediated intracellular Ca2+ influx and the nuclear factor-kappa B (NF-kappa B) pathway, as well as the phosphorylation of mitogen-activated protein (MAP) kinases. Although OC did not inhibit the phosphorylation of Fyn, Lyn, and Syk, it directly inhibited the tyrosine kinase activity in vitro. Moreover, oral administration of OC inhibited the IgE-induced PSA reaction in a dose-dependent manner. Taken together, the present study provides new insights into the anti-allergic activity of OC, which could be a promising candidate for allergic therapy.
[1]Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China.
[2]Shanghai Coll TCM New Drug Discovery, Engn Res Ctr, Shanghai 201203, Peoples R China.
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