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Paeoniflorin exerts a nephroprotective effect on concanavalin A-induced damage through inhibition of macrophage infiltration 期刊论文

编号：

ff43062e-2834-437d-8d48-028b4fc77eae
作者：

Liu, Cheng(刘铖)#^[1]Cheng, Zhuoan^[1];Wang, Yunman^[2];Dai, Xiuqin^[1];Zhang, Jie(张洁)*^[3]Xue, Dongying(薛冬英)^[3]
语种：

英文
期刊：

DIAGNOSTIC PATHOLOGY ISSN：1746-1596 2015 年 10 卷 ; JUL 25
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关键词：

Paeoniflorin CXCR3-CXCL11 Macrophage infiltration Concanavalin A
摘要：

Background: It is well established that macrophage infiltration is involved in concanavalin A (conA)-induced liver injury. However, the role of macrophages in conA-induced renal injury remains unknown. The aims of this study were to investigate macrophage infiltration in conA-induced renal injury and determine whether paeoniflorin (PF) could inhibit macrophage infiltration into the kidney.
Methods: BALB/C mice were pre-treated with or without PF 2 h (h) before conA injection. At 8 h after con A injection, all the mice were sacrificed; The liver and kidney histology were studied. The renal CD68 expression was detected by immunohistochemical and real-time PCR analysis. The level of expression of C-X-C chemokine receptor type 3 (CXCR3) was analyzed by western blot, immunohistochemical and real-time PCR. The pathophysiological involvement of CXCR3 in macrophage infiltration were investigated using dual-colour immunofluorescence microscopy.
Results: PF administration significantly reduced the elevated serum levels of alanine transaminase (ALT), blood urea nitrogen (BUN), creatinine (Cr) and the severity of liver and renal damage compared with that in the conA-vehicle group. PF administration inhibited the increase in renal IL1 beta mRNA expression and concentration. Furthermore, immunohistochemical analysis showed that macrophages secreted CXCR3 in the kidneys of the conA-vehicle mice. Immunofluorescence microscopy demonstrated CXCR3 bound tightly to C-X-C motif ligand 11 (CXCL11) in the kidneys of the conA-vehicle mice and showed that PF treatment could suppress CXCR3/CXCL11 over-activation.
Conclusions: Macrophage infiltration was a notable pathological change in the kidneys of conA-treated mice. PF administration attenuated conA-induced renal damage, at least in part, by inhibiting the over-activated CXCR3/CXCL11 signal axis.
推荐引用方式
GB/T 7714：

Liu Cheng,Cheng Zhuoan,Wang Yunman, et al. Paeoniflorin exerts a nephroprotective effect on concanavalin A-induced damage through inhibition of macrophage infiltration [J].DIAGNOSTIC PATHOLOGY,2015,10.
APA：

Liu Cheng,Cheng Zhuoan,Wang Yunman,Dai Xiuqin,&Xue Dongying.(2015).Paeoniflorin exerts a nephroprotective effect on concanavalin A-induced damage through inhibition of macrophage infiltration .DIAGNOSTIC PATHOLOGY,10.
MLA：

Liu Cheng, et al. "Paeoniflorin exerts a nephroprotective effect on concanavalin A-induced damage through inhibition of macrophage infiltration" .DIAGNOSTIC PATHOLOGY 10(2015).
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