Mossy fiber sprouting (MFS) is a pathological phenomenon that is commonly observed in temporal lobe epilepsy (TLE). However, the molecular mechanisms underlying MFS remain unclear. It has been demonstrated that the tau protein is important in the progression of MFS by the regulation of microtubule dynamics and axonal transport, with all of these functions of tau modulated by its site-specific phosphorylation. Glycogen synthase kinase-3 beta (GSK-3 beta) is an active kinase that regulates the phosphorylation of tau protein. Therefore, it was hypothesized that GSK-3 beta contributes to MFS by phosphorylating tau protein. The aim of the present study was to determine the expression and activity of GSK-3 beta at different regions in the rat hippocampus during the pentylenetetrazole (PTZ)-kindling process in order to demonstrate the possible correlation with MFS, and to investigate the involvement of GSK-3 beta in epileptogenesis. Male Sprague-Dawley rats (n=180) were randomly divided into the control and PTZ-treated groups. The chronic epileptic model was established by intraperitoneal injection of PTZ and the hippocampus was observed for the presence of MFS using Timm staining. GSK-3 beta mRNA, protein and activity were analyzed in various regions of the hippocampus using in situ hybridization, immunohistochemistry and immunoprecipitation followed by a kinase assay and liquid scintillation counting, respectively. MFS was observed prior to kindling and an increased distribution of Timm granules were observed in the CA3 region of the PTZ-treated rats; however, this was not demonstrated in the supragranular layer of the dentate gyrus. The expression of GSK-3 beta mRNA and protein, as well as the GSK-3 beta activity, increased significantly from 3 days to 4 weeks in the PTZ group, and this was correlated with the progression of MFS in the CA3 area. In addition, it was demonstrated that MFS did not result from TLE. GSK-3 beta may therefore be involved in the progression of MFS and is important in epileptogenesis. An understanding of the molecular mechanisms underlying MFS may lead to the identification of a novel therapeutic target to limit epileptogenesis.
[1]Cent S Univ, Xiangya Hosp, Dept Neurol, Changsha 410008, Hunan, Peoples R China.
[2]Putuo Hosp Shanghai, Dept Neurol, Shanghai 200062, Peoples R China.
[3]Fujian Med Univ, Zhangzhou Hosp, Dept Neurol, Zhangzhou 363000, Fujian, Peoples R China.
[4]Sanbofuxing Brain Hosp, Dept Neurol, Beijing 100038, Peoples R China.
[5]1st Peoples Hosp Zhengzhou, Dept Neurol, Zhengzhou 450004, Henan, Peoples R China.
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