Eight isovaleryl sucrose esters, named ainslosides A-H (1-8), were isolated from Ainsliaea yunnanensis Franch. Their structures, including the absolute configurations of the sugar residues, were elucidated by extensive analysis of NMR spectra and acid hydrolysis. All compounds were tested in vitro for cytotoxicity against four human tumour cell lines, A549, HCT116, MDA-MB-231, and BEL7404. Among the compounds tested, ainsloside B (2) showed potent cytotoxicity against the A549 cell line with an IC50 value of 3.3 mu M. Flow cytometry analysis showed that compound 2 can arrest the cell cycle at the G(0)/G(1) phase and induce cell apoptosis in A549 cells. Further studies indicated that the apoptosis-inducing effect of compound 2 may be involved in the reduction of mitochondrial membrane potential (MMP) and increase of reactive oxygen species (ROS) level in A549 cells.
[1]Second Mil Med Univ, Sch Pharm, Dept Phytochem, Shanghai 200433, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China.
[3]Shanghai Inst Pharmaceut Ind, Shanghai 200400, Peoples R China.
(8.0+极速模式)