Although 5-year survival rate of non-metastatic colorectal cancer (CRC) is high, about 10% of patients in stage I and II still develop into metastatic CRC and eventually die after resection. Currently, there is no effective biomarker for predicting the prognosis of non-metastatic CRC in clinical practice. In this study, we identified miR-650 as a biomarker for prognosis prediction. We observed that the expression of miR-650 in tumor tissues had a positive association with overall survival. MiR-650 inhibited cell growth and invasion in vitro and in vivo. Furthermore, miR-650 targeted AKT2 and repressed the activation of the AKT pathway (AKT2/GSK3 beta/E-cadherin). Thus it induced the translocation of E-cadherin and beta-catenin in cancer cells. Our results highlight the potential of miR-650 as a prognostic prediction biomarker and therapeutic target in non-metastatic CRC via inhibition of the AKT2/GSK3 beta/E-cadherin pathway.
[1]Fudan Univ, Sch Basic Med Sci, Dept Pathol, Shanghai, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Dept Pathol, Shanghai, Peoples R China.
[3]Harvard Med Sch, Brigham & Women Med, Div Surg Oncol, Boston, MA USA.
(8.0+极速模式)