Objective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system. Background Data. Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy. Methods. Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2). After irradiation by 670nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured. Results. The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells. Conclusion. 670nm LED irradiation could recover the hypoxia damage caused by CoCl2. Photobiomodulation of 670nm LED plays a potential role for the treatment of diabetic retinopathy.
[1]Univ Shanghai Sci & Technol, Coll Med Device & Food Engn, Shanghai 200093, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China.
[3]Chinese Acad Sci, Key Lab Syst Biol, Shanghai 201210, Peoples R China.
[4]Chinese Acad Sci, Shanghai Adv Res Inst, Lab Syst Biol, Shanghai 201210, Peoples R China.
(8.0+极速模式)