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Heat induces adenosine triphosphate release from mast cells in vitro: a putative mechanism for moxibustion 期刊论文

编号：

e5bf894e-16a1-4663-b9d1-bcd32c2ccb74
作者：

Hu Lei#^[1,2]Wang Lina(汪丽娜)*^[1,2]Wei Jianzi(魏建子)^[1]Grygorczyk, Ryszard^[4,5];Shen Xueyong(沈雪勇)^[1,2]Schwarz, Wolfgang^[2,3];
语种：

英文
期刊：

JOURNAL OF TRADITIONAL CHINESE MEDICINE ISSN：0255-2922 2015 年 35 卷 3 期 (323 - 328) ; JUN 15
收录：
关键词：

Fire toxin syndrome Mast cells Cell degranulation Calcium Adenosine triphosphate Moxibustion
摘要：

OBJECTIVE:To investigate the role of adenosine triphosphate (ATP) purinergic signaling in mast cells (MCs) modulated by heat to further understand the molecular mechanisms of moxibustion.
METHODS: Skin temperatures induced by monks-hood cake moxibustion were evaluated by measuring the Neiguan acupoint (PC 6) from 31 participants with a digital thermocouple thermometer. Temperatures of 43 degrees C and 52 degrees C were applied to cultured human leukemia mast cell line HMC-1 in vitro. Calcium fluorescence was applied to detect intracellular Ca2+ ([Ca2+](i)). Extracellular ATP contents were measured by luciferin-luciferase assay.
RESULTS: Maximum skin temperatures mostly ranged from 40-45 C degrees, but some reached up to 50 degrees C. Both 43 degrees C and 52 degrees C induced MC degranulation, which was accompanied by an increase in [Ca2+](i) and ATP release. Complexing extracellular Ca2+ with 5 mM ethylene glycol-bis (B-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) inhibited the noxious heat-induced elevation of [Ca2+](i), and prevented the enhanced ATP secretion by those cells at 52 degrees C, but not 43 degrees C.
CONCLUSION: Monkshood cake moxibustion can generate heat sufficient to trigger cellular events of MCs, including degranulation, [Ca2+](i), elevation, and ATP release, suggesting that purinergic signals originating from MCs are possibly the initiating response of acupoints to moxibustion. (C) 2015 JTCM. All rights reserved.
推荐引用方式
GB/T 7714：

Hu Lei,Wang Lina,Wei Jianzi, et al. Heat induces adenosine triphosphate release from mast cells in vitro: a putative mechanism for moxibustion [J].JOURNAL OF TRADITIONAL CHINESE MEDICINE,2015,35(3):323-328.
APA：

Hu Lei,Wang Lina,Wei Jianzi,Grygorczyk Ryszard,&Schwarz Wolfgang.(2015).Heat induces adenosine triphosphate release from mast cells in vitro: a putative mechanism for moxibustion .JOURNAL OF TRADITIONAL CHINESE MEDICINE,35(3):323-328.
MLA：

Hu Lei, et al. "Heat induces adenosine triphosphate release from mast cells in vitro: a putative mechanism for moxibustion" .JOURNAL OF TRADITIONAL CHINESE MEDICINE 35,3(2015):323-328.
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