Alzheimer disease (AD) is characterized by the accumulation of amyloid- (A) protein and intracellular neurofibrillary tangles. Previous studies have shown that A aggregation is one of the most important initiating factors in the pathogenesis of AD. Oligomers of A cause neurotoxicity, synaptic dysfunction and memory impairments that underlie AD. An increasing number of studies have shown that oligomeric A may bind with a number of surface proteins to mediate its neuronal toxicity. Previously, it was shown that ATP synthase is present on the cell surface and binds with oligomeric A. In the present study, ATP synthase was confirmed to be present on the surface of neurons and oligomeric A was observed to induce neuron damage and expression of amyloid precursor protein (APP) and Fe65 increase. Results showed that inhibition of surface ATP synthase may reduce the neuronal damage by LDH release assay and decrease APP and Fe65 expression by immunofluorescence and western blot analysis. These results confirmed that the cell surface ATP synthase is a binding protein for A on neural cells and suggested that the surface ATP synthase may be involved in the neurotoxic effects of oligomeric A and may be an intervening target of pathogenesis of AD.
[1]Shanghai Univ Tradit Chinese Med, Shanghai Geriatr Inst Chinese Med, Shanghai 200031, Peoples R China.
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