Objective. To explore the effects of puerarin to treat endometriosis (EMT) model rats and the possible regulatory mechanisms. Methods. EMT model rats were surgically induced by autotransplantion of endometrial tissues. The appropriate dosage of puerarin to treat EMT model rats was determined by observing the pathologic morphology of ectopic endometrial tissues and by detecting the levels of estradiol (E2) and prostaglandin E2 (PGE2) of both serum and ectopic endometrial tissues. The related genes and proteins of ectopic endometrial tissues were analyzed by Real-time PCR and immunohistochemistry (IHC) to explore the possible mechanisms. Results. Puerarin could reduce the levels of E2 and PGE2 and prevent the growth of ectopic endometrium tissues by inhibiting the expression of aromatase cytochrome P450 (p450arom) and cyclooxygenase-2 (cox-2); puerarin could adjust the anabolism of E2 by upregulating the expression of 17 beta-hydroxysteroid-2 (17 beta-hsd-2) and downregulating the expression of 17 beta-hydroxysteroid-1 (17 beta-hsd-1) of the ectopic endometrium tissues; puerarin could increase the expression of ER.. and improve the inflammatory microenvironment of EMT model rats. Conclusions. Our data suggest that puerarin has a therapeutic effect on EMT model rats and could be a potential therapeutic agent for the treatment of EMT in clinic.
[1]Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China.
[2]Second Mil Med Univ, Changhai Hosp, Dept Gynecol Tradit Chinese Med, Shanghai 200433, Peoples R China.
[3]Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Dept Gynecol Tradit Chinese Med, Longhua Hosp, Shanghai 200032, Peoples R China.
(8.0+极速模式)