Excessive autophagy plays a crucial role in cigarette smoking extract (CSE)-induced inflammation response and oxidative damage of respiratory epithelial cells. The components from Eclipta prostrata L. (CCE) have been shown to be beneficial for CSE-induced epithelial cells injury. However, whether its protection on CSE-stress injury is related to its regulation on autophagy remains still unclear. In this study, CCE, containing mainly wedelolactone of 45.88% and demethylwedelolactone of 23.74%, could improve significantly 10% CSE-induced cell viability of normal human bronchial epithelial (NHBE) cells using CCK-8 kit. We revealed that CCE could remarkably increase autophagic factors Beclin-1, Atg5, ATF4 proteins expression levels and the transformation of LC3-I to LC3-II. Additionally, CCE up-regulated significantly p-p16 and p-p21 phosphorylation levels whereas down-regulated p-p53 in NHBE cells. The changes of typical autolysosom and representative autophagosome in the presence of CCE or/and autophagy inhibitor chloroquine (CQ) were also observed by transmission electron microscopy. These data demonstrated that CCE reduced CSE-induced autophagy flux activation in NHBE cells. The blockade of CCE on autophagy flux contributes to its protection against CSE-induced NHBE cells damage, and CCE is promising to be combination therapeutic molecules to excessive autophagic damage in respiratory diseases.
[1]Changzhou Univ, Sch Pharmaceut Engn & Life Sci, Changzhou, Peoples R China.
[2]Jiangsu Prov Acad Chinese Med, Key Lab New Drug Delivery Syst Chinese Mat Med, Nanjing, Jiangsu, Peoples R China.
[3]Anhui Univ Chinese Med, Coll Tradit Chinese Med, Hefei, Anhui, Peoples R China.
[4]Minzu Univ China, China Minor Tradit Med Ctr, Beijing, Peoples R China.
[5]China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing, Jiangsu, Peoples R China.
[6]Shanghai Univ Chinese Med, Affliated Hosp Long Hua, Shanghai, Peoples R China.
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