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Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography 期刊论文

编号：

d29f2922-099f-45b9-bc87-c8d34f11c308
作者：

Lu, Lu#^[1]Liu, Yanping^[1];Ding, Yue(丁越)^[2]Hou, Jianwei(侯剑伟)*^[2]Zhang, Yong^[2];Xue, Haiping(薛海萍)^[2]Zhang, Tong(张彤)*^[2]
语种：

英文
期刊：

NATURAL PRODUCT RESEARCH ISSN：1478-6419 2016 年 30 卷 20 期 (2364 - 2367)
收录：
关键词：

-D-glycosidase enzymolysis preparative liquid chromatography timosaponin AIII
摘要：

Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed. -D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7g of TAIII with a high purity of > 97% was obtained from 1kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale.
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推荐引用方式
GB/T 7714：

Lu Lu,Liu Yanping,Ding Yue, et al. Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography [J].NATURAL PRODUCT RESEARCH,2016,30(20):2364-2367.
APA：

Lu Lu,Liu Yanping,Ding Yue,Hou Jianwei,&Zhang Tong.(2016).Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography .NATURAL PRODUCT RESEARCH,30(20):2364-2367.
MLA：

Lu Lu, et al. "Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography" .NATURAL PRODUCT RESEARCH 30,20(2016):2364-2367.
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