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Hepatoprotective effect of Forsythiae Fructus water extract against carbon tetrachloride-induced liver fibrosis in mice 期刊论文

编号：

d0fc9728-6250-4a6e-ae24-1ba0ee73ff5f
作者：

Zhang, Yi#^[1,2]Miao, Hui^[1,2];Yan, Hongyu^[1,2];Sheng, Yuchen(盛雨辰)^[3]Ji, Lili(季莉莉)*^[1,2]
语种：

英文
期刊：

JOURNAL OF ETHNOPHARMACOLOGY ISSN：0378-8741 2018 年 218 卷 (27 - 34) ; MAY 23
收录：
关键词：

FSE Liver fibrosis Collagen HSCs EMT
摘要：

Ethnopharmacological relevance: The fruit of Forsythia suspensa (Thunb.) Vahl, named Forsythiae Fructus (Lian-Qiao), is a well-known traditional Chinese medicine (TCM) used for clearing away heat and toxic material, eliminating the mass and relieving swelling.
Aim of the study: This study aims to observe the attenuation of the water extract of Forsythiae Fructus (FSE) on carbon tetrachloride (CCl4)-induced hepatic fibrosis in male C57BL/6 mice.
Materials and methods: Hepatic fibrosis was induced in male C57BL/6 mice by intraperitoneal injection with 2 ml/kg CCl4(mixed 1: 3 in olive oil) twice a week for 4 weeks. At the same time, the mice were orally given with FSE (1, 2 g/kg) every day for 4 weeks. Serum biochemical parameters, gene and protein expression related to liver fibrosis were analyzed. The contents of forsythiaside A and forsythin in FSE were measured by high-performance liquid chromatography (HPLC).
Results: Results of serum alanine/aspartate aminotransferase (ALT/AST) activity and liver histological evaluation both showed the protection of FSE against CCl4-induced liver injury. Further, the anti-fibrotic effects of FSE was evidenced by the results of Masson's trichrome and Sirius red staining, liver hydroxyproline content, and serum amounts of hyaluronic acid, laminin, collagen IV and type III procollagen (PCIII). FSE also reduced the expression of alpha-smooth muscle actin (alpha-SMA) in livers from CCl4-injured mice. Additionally, FSE decreased the increased hepatic expression of fibroblast-specific protein 1 (FSP1) and vimentin induced by CCl4 in mice.
Conclusions: FSE attenuates CCl4-induced liver fibrosis in mice by inhibiting hepatic stellate cells (HSCs) activation, reducing hepatic extracellular matrix (ECM) disposition and reversing epithelial-mesenchymal transition (EMT).
推荐引用方式
GB/T 7714：

Zhang Yi,Miao Hui,Yan Hongyu, et al. Hepatoprotective effect of Forsythiae Fructus water extract against carbon tetrachloride-induced liver fibrosis in mice [J].JOURNAL OF ETHNOPHARMACOLOGY,2018,218:27-34.
APA：

Zhang Yi,Miao Hui,Yan Hongyu,Sheng Yuchen,&Ji Lili.(2018).Hepatoprotective effect of Forsythiae Fructus water extract against carbon tetrachloride-induced liver fibrosis in mice .JOURNAL OF ETHNOPHARMACOLOGY,218:27-34.
MLA：

Zhang Yi, et al. "Hepatoprotective effect of Forsythiae Fructus water extract against carbon tetrachloride-induced liver fibrosis in mice" .JOURNAL OF ETHNOPHARMACOLOGY 218(2018):27-34.
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