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MicroRNA Expression Patterns Involved in Amyloid Beta-Induced Retinal Degeneration 期刊论文

编号：

c98cc7a5-698f-461d-ac66-c1be015fa77d
作者：

Huang, Peirong#^[1,2]Sun, Junran#^[1,2]Wang, Fenghua^[1,2];Luo, Xueting^[3];Feng, Jingyang^[1,2];Gu, Qing^[2];Liu, Te(刘特)*^[4]Sun, Xiaodong*^[1,2,3]
语种：

英文
期刊：

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE ISSN：0146-0404 2017 年 58 卷 3 期 (1726 - 1735) ; MAR
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关键词：

amyloid beta retina microRNA age-related macular degeneration
摘要：

PURPOSE. Dry age-related macular degeneration (AMD) is characterized by the accumulation of drusen under Bruch's membrane, and amyloid beta (A beta) is speculated to be one of the key pathologic factors. While the detrimental effects of A beta on retinas have been widely explored, A beta-induced epigenetic regulatory changes have yet to be fully investigated. We therefore aimed to identify the microRNA (miRNA) expression profiles in an A beta-induced mouse model of retinal degeneration.
METHODS. C57BL/6 mice were intravitreally injected with A beta(1-40) or PBS and the eye tissues were collected for hematoxylin and eosin (H&E) staining, apoptosis immunofluorescence staining, and miRNA profiling. After filtering, 10 miRNAs and their target genes were chosen for quantitative RT-PCR (qRT-PCR) confirmations. Pathway analyses were employed for further bioinformatic analyses.
RESULTS. Hematoxylin and eosin-stained sections of retinal pigment epithelium (RPE)/neural retina tissue demonstrated degenerative alterations, and immunofluorescence testing revealed apoptosis within the retina after A beta treatments. MicroRNA profiling revealed 61 miRNAs that were differentially expressed between the model and the control group. Among these, 38 miRNAs were upregulated (fold change > 1.5, P < 0.05) and 23 miRNAs were downregulated (fold change < 0.667, P < 0.05). Five of the 10 selected miRNAs (miR-142, miR-216, miR-155, miR-223, and miR-433) as well as several key target genes (CFH, IGF-1R, c-MET, and ABCA1) were confirmed by qRT-PCR analyses.
CONCLUSIONS. Our study is the first to profile the miRNA expression patterns and suggests that A beta accumulation could lead to relevant biochemical alternations such as complement activation, barrier impairment, apoptosis, and positive feedback of A beta production.
推荐引用方式
GB/T 7714：

Huang Peirong,Sun Junran,Wang Fenghua, et al. MicroRNA Expression Patterns Involved in Amyloid Beta-Induced Retinal Degeneration [J].INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2017,58(3):1726-1735.
APA：

Huang Peirong,Sun Junran,Wang Fenghua,Luo Xueting,&Sun Xiaodong.(2017).MicroRNA Expression Patterns Involved in Amyloid Beta-Induced Retinal Degeneration .INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,58(3):1726-1735.
MLA：

Huang Peirong, et al. "MicroRNA Expression Patterns Involved in Amyloid Beta-Induced Retinal Degeneration" .INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 58,3(2017):1726-1735.
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