The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo co-culture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-beta, peroxisome proliferator-activated receptor-gamma, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology.
[1]Shanghai Univ Tradit Chinese Med, PuTuo Hosp, Dept Nucl Med, Shanghai 200062, Peoples R China.
[2]Second Mil Med Univ, Res Ctr Dev Biol, Shanghai 200433, Peoples R China.
[3]Second Mil Med Univ, Changhai Hosp, Lab Diag Dept, Shanghai 200433, Peoples R China.
(8.0+极速模式)