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The combination of Ilexhainanoside D and ilexsaponin A(1) reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease 期刊论文

编号：

bec363f8-c32c-44bf-a042-fba4be784ce8
作者：

Zhao, Wenwen#^[1]Xiao, Meng^[1];Yang, Jie^[2];Zhang, Li(张莉)^[3]Ba, Yinying^[1];Xu, Rongrong^[1];Liu, Zongyang^[1];Zou, Haiyan^[1];Yu, Ping^[1];Wu, Xia*^[1]Chen, Xiaoqing*^[1]
语种：

英文
期刊：

PHYTOMEDICINE ISSN：0944-7113 2019 年 63 卷 ; OCT
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关键词：

Ilex hainanensis Triterpenoid saponins Non-alcoholic fatty liver disease Gut microbiota Inflammation Intestinal barrier
摘要：

Background: Non-alcoholic fatty liver disease (NAFLD) is becoming a major health concern worldwide. Ilex hainanensis Merr. extract was proved to have anti-inflammation effect on NAFLD, and Ilexhainanoside D (IhD) and ilexsaponin A(1) (IsA) were the main triterpenoid saponins extracted from it.
Purposes: To investigate the hepatoprotective effect of the combination of IhD and IsA (IIC) against NAFLD and discuss the potential mechanisms.
Methods: Male C57BL/6 mice were fed a high-fat diet (HFD) to induce NAFLD and were treated with IIC (60, 120 or 240 mg/kg) for 8 weeks. Growth parameters, abdominal fat content, serum biochemical markers, hepatic lipid accumulation and insulin tolerance were assessed. Quantitative real-time PCR was used to determine the hepatic gene expression of TLR2, TLR4, TNF-alpha, IL-6, and IL-1 beta. Western blot analysis was performed to determine the expression of the epidermal tight junction proteins ZO-1 and occludin. Gut microbiota profiles were established via high-throughput sequencing of the V3-V4 region of the bacterial 16S rRNA gene.
Results: IIC significantly reduced the severity of NAFLD induced by HFD in a dose-dependent manner. IIC decreased the ratio of Firmicutes/Bacteroidetes, reduced the relative abundance of Desulfovibrio and increased the relative abundance of Akkerrnansia. The intestinal barrier was improved as evidenced by the upregulation of the expression of ZO-1 and occludin in the ileum. IIC thus reduced the entry of LPS into the circulation and decreased the hepatic gene expression levels of proinflammatory cytokines.
Conclusion: This approach demonstrated the positive effects of IIC in a mouse model of NAFLD, indicating that it possibly acts by reducing inflammation and improving the intestinal barrier function.
推荐引用方式
GB/T 7714：

Zhao Wenwen,Xiao Meng,Yang Jie, et al. The combination of Ilexhainanoside D and ilexsaponin A(1) reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease [J].PHYTOMEDICINE,2019,63.
APA：

Zhao Wenwen,Xiao Meng,Yang Jie,Zhang Li,&Chen Xiaoqing.(2019).The combination of Ilexhainanoside D and ilexsaponin A(1) reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease .PHYTOMEDICINE,63.
MLA：

Zhao Wenwen, et al. "The combination of Ilexhainanoside D and ilexsaponin A(1) reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease" .PHYTOMEDICINE 63(2019).
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