Portulaca oleracea L. is a traditional Chinese medicine, which has been used as adjuvant therapy for inflammatory bowel disease (IBD). However, the mechanism of its activity in IBD still remains unclear. Since previous studies have documented the anti-inflammatory effect of peroxisome proliferator activated receptors-gamma (PPAR-gamma), Portulaca regulation of PPAR-gamma in inflammation was examined in current study. Ulcerative colitis (UC) was generated by 5% dextran sulfate sodium (DSS) in mice and four groups were established as normal control, DSS alone, DSS plus mesalamine, and DSS plus Portulaca. Severity of UC was evaluated by body weight, stool blood form, and length of colorectum. Inflammation was examined by determination of inflammatory cytokines (TNF-a, IL-6, and IL-1a). Portulaca extract was able to attenuate development of UC in DSS model similar to the treatment of mesalazine. Moreover, Portulaca extract inhibited proinflammatory cytokines release and reduced the level of DSS-induced NF-kappa B phosphorylation. Furthermore, Portulaca extract restored PPAR-gamma level, which was reduced by DSS. In addition, Portulaca extract protected DSS induced apoptosis in mice. In conclusion, Portulaca extract can alleviate colitis in mice through regulation of inflammatory reaction, apoptosis, and PPAR-gamma level; therefore, Portulaca extract can be a potential candidate for the treatment of IBD.
[1]Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China.
[2]Soochow Univ, Sch Med, Suzhou 215006, Peoples R China.
[3]Nanjing Med Univ, Sch Clin Med, Shanghai Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Dept Immunol & Microbiol, Shanghai 201203, Peoples R China.
[5]Wenzhou Med Univ, Eye Hosp, Wenzhou City 325027, Zhejiang, Peoples R China.
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