Low-level-laser therapy (LLLT) is an effective complementary treatment, especially for anti-inflammation and wound healing in which dermis or mucus mast cells (MCs) are involved. In periphery, MCs crosstalk with neurons via purinergic signals and participate in various physiological and pathophysiological processes. Whether extracellular ATP, an important purine in purinergic signaling, of MCs and neurons could be modulated by irradiation remains unknown. In this study, effects of red-laser irradiation on extracellular ATP content of MCs and dorsal root ganglia (DRG) neurons were investigated and underlying mechanisms were explored in vitro. Our results show that irradiation led to elevation of extracellular ATP level in the human mast cell line HMC-1 in a dose-dependent manner, which was accompanied by elevation of intracellular ATP content, an indicator for ATP synthesis, together with [Ca2+](i) elevation, a trigger signal for exocytotic ATP release. In contrast to MCs, irradiation attenuated the extracellular ATP content of neurons, which could be abolished by ARL 67156, a nonspecific ecto-ATPases inhibitor. Our results suggest that irradiation potentiates extracellular ATP of MCs by promoting ATP synthesis and release and attenuates extracellular ATP of neurons by upregulating ecto-ATPase activity. The opposite responses of these two cell types indicate complex mechanisms underlying LLLT.
[1]Shanghai Univ Tradit Chinese Med, Acupuncture & Moxibust Coll, Shanghai 201203, Peoples R China.
[2]Shanghai Res Ctr Acupuncture & Meridians, Shanghai 201203, Peoples R China.
[3]Ctr Hosp Univ Montreal CRCHUM, Res Ctr, Montreal, PQ H2X 0A9, Canada.
[4]Univ Montreal, Dept Med, Montreal, PQ H3C 3T5, Canada.
[5]Goethe Univ Frankfurt, Inst Biophys, D-60438 Frankfurt, Germany.
(8.0+极速模式)