EEG analysis is a traditional method used for acupuncture manipulation analyzing. In this paper, Cross-correlation method was used to process the signals obtained from a carefully designed acupuncture experiment. The main objective of this work is to find the influence of acupuncture for brain. A protocol composed of three different manipulations has been designed to acquire the EEG signals using 22 biosensors. Totally 6 healthy volunteers with an age group of 23-27 years old have been used in this acupuncture experiment and cross-correlation method was adopt to reveal the differences among several acupuncture stages. An apparent enhancement was found in the study that the synchronization of EEG when during acupuncture.