Mycoplasma pneumoniae pneumonia (MPP) is the most common respiratory infection in young children and its incidence has increased worldwide. In this study, high expression of chemokine ligand 5 (CCL5) was observed in the serum of MPP patients, and its expression was positively correlated to DNA of M. pneumoniae (MP-DNA). In vitro, M. pneumoniae (MP) infection to A549 cells induced the expression of CCL5, chemokines receptor 4 (CCR4), nuclear factor-kappa B (NF-kappa B) nuclear protein, and phosphorylation of NF-kappa B-p65 (p-NF-kappa B-p65), whereas NF-kappa B cytoplasmic protein was decreased. On the contrary, treatment of hyperoside counteracted the induction of MP infection and promoted the proliferation of MP-infected A549 cells. Similarly, MP-induced IL-8 and TNF-alpha production was also markedly reduced by hyperoside. And CCR4 inhibitor AZD2098 had a better effect than hyperoside. In addition, CCL5 recombinant protein inhibited the effect of hyperoside to promote IL-8 and TNF-alpha production and CCR4 expression. These results indicated that CCL5 may be involved in the progression of MPP, and hyperoside was beneficial for MPP probably through CCL5-CCR4 interactions, which may provide a potential effective therapy for MPP.
[1]Tongji Univ, Dept Pediat, Affiliated East Hosp, Shanghai 200123, Peoples R China.
[2]Shanghai Municipal Hosp Tradit Chinese Med, Dept Pediat, Shanghai 200071, Peoples R China.
[3]Shanghai Jiao Tong Univ, Sch Pharm, 800 DongChuan Rd, Shanghai 200240, Peoples R China.
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