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Increased Expression of ERp57 in Rat Oocytes During Meiotic Maturation Is Associated With Sperm -Egg Fusion 期刊论文

编号：

a3b20846-b478-40f2-99e1-d9792597f66a
作者：

Liu, Yue#^[1]Zhu, Yemin^[2];Wu, Xiaohui(吴晓慧)^[3]Li, Yandong^[4];Guo, Qiangsu^[1];Li, Weiping^[5];Ding, Zhide*^[1]
语种：

英文
期刊：

MOLECULAR REPRODUCTION AND DEVELOPMENT ISSN：1040-452X 2014 年 81 卷 4 期 (315 - 325) ; APR
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摘要：

Oocyte meiotic maturation is a developmental transition that starts during germinal-vesicle breakdown and ends at the arrest in metaphase of meiosis II. This transition is associated with changes to both the proteins that are synthesized and the abundance/distribution of post-translational modifications that are crucial for subsequent fertilization and embryogenesis. Here, we isolated and cultured rat oocytes in vitro during both metaphase of meiosis I (MI) and meiosis II (MII) stages, respectively, and then compared their proteomic profiles by high-resolution, two-dimensional gel electrophoresis (2DE) followed by mass spectrometry. We found that the expression of five proteins was up-regulated while six proteins were down-regulated when comparing MI to MII oocytes. The expression of ERp57, an endoplasmic reticulum chaperone, underwent a dramatic increase between MI and MII oocytes, and became concentrated in a dome-shaped area of the cell surface within the microvillar region. A similar profile was observed during spermatogenesis, and sperm ERp57 eventually localized to the head and flagellum surfaces, finally ending in the equatorial region of acrosome-reacted sperm. Given the localization pattern, we tested and found that a polyclonal antiserum created against recombinant rat ERp57 significantly inhibited spermatozoa from penetrating zona pellucida-free oocytes without affecting either sperm motility or the acrosome reaction. These results indicate that ERp57 expression on oocytes, and possibly sperm, plays an important physiological role during sperm-egg fusion. Mol. Reprod. Dev. 81: 315-325, 2014. (c) 2014 Wiley Periodicals, Inc.
推荐引用方式
GB/T 7714：

Liu Yue,Zhu Yemin,Wu Xiaohui, et al. Increased Expression of ERp57 in Rat Oocytes During Meiotic Maturation Is Associated With Sperm -Egg Fusion [J].MOLECULAR REPRODUCTION AND DEVELOPMENT,2014,81(4):315-325.
APA：

Liu Yue,Zhu Yemin,Wu Xiaohui,Li Yandong,&Ding Zhide.(2014).Increased Expression of ERp57 in Rat Oocytes During Meiotic Maturation Is Associated With Sperm -Egg Fusion .MOLECULAR REPRODUCTION AND DEVELOPMENT,81(4):315-325.
MLA：

Liu Yue, et al. "Increased Expression of ERp57 in Rat Oocytes During Meiotic Maturation Is Associated With Sperm -Egg Fusion" .MOLECULAR REPRODUCTION AND DEVELOPMENT 81,4(2014):315-325.
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