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Improved TLC Bioautographic Assay for Qualitative and Quantitative Estimation of Tyrosinase Inhibitors in Natural Products 期刊论文

编号：

9fac7ec3-a815-4bc3-9c51-c292f175cf75
作者：

Zhou, Jinge#^[2,3]Tang, Qingjiu^[4];Wu, Tao(吴弢)*^[2]Cheng, Zhihong*^[1]
语种：

英文
期刊：

PHYTOCHEMICAL ANALYSIS ISSN：0958-0344 2017 年 28 卷 2 期 (115 - 124) ; MAR-APR
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关键词：

Rhodiola sacra tyrosinase inhibito TLC bioautography kojic acid
摘要：

Introduction - TLC bioautography for tyrosinase inhibitors has made recent progress; however, an assay with a relative low consumption of enzyme and quantitative capability would greatly advance the efficacy of related TLC bioautographic assays.
Objective - An improved TLC bioautographic assay for detecting tyrosinase inhibitors was developed and validated in this study.
Methods - L-DOPA (better water-solubility than L-tyrosine) was used as the substrate instead of reported L-tyrosine. The effects of enzyme and substrate concentrations, reaction temperatures and times, and pH values of the reaction system as well as different plate types on the TLC bioautographic assay were optimised. The quantitative analysiswas conducted by densitometric scanning of spot areas, and expressed as the relative tyrosinase inhibitory capacity (RTIC) using a positive control (kojic acid) equivalent.
Results - The limit of detection (LOD) of this assay was 1.0 ng for kojic acid. This assay has acceptable accuracy (101.73-102.90%), intra- and inter-day, and intra- and inter-plate precisions [relative standard deviation (RSD), less than 7.0%], and ruggedness (RSD, less than 3.5%). The consumption of enzyme (75 U/mL) is relatively low. Two tyrosinase inhibitory compounds including naringenin and 1-O-beta-D-glucopyranosyl-4-allylbenzene have been isolated from Rhodiola sacra guided by this TLC bioautographic assay.
Conclusion - Our improved assay is a relatively low-cost, sensitive, and quantitative method compared to the reported TLC bioautographic assays. Copyright (C) 2016 John Wiley & Sons, Ltd.
推荐引用方式
GB/T 7714：

Zhou Jinge,Tang Qingjiu,Wu Tao, et al. Improved TLC Bioautographic Assay for Qualitative and Quantitative Estimation of Tyrosinase Inhibitors in Natural Products [J].PHYTOCHEMICAL ANALYSIS,2017,28(2):115-124.
APA：

Zhou Jinge,Tang Qingjiu,Wu Tao,Cheng Zhihong.(2017).Improved TLC Bioautographic Assay for Qualitative and Quantitative Estimation of Tyrosinase Inhibitors in Natural Products .PHYTOCHEMICAL ANALYSIS,28(2):115-124.
MLA：

Zhou Jinge, et al. "Improved TLC Bioautographic Assay for Qualitative and Quantitative Estimation of Tyrosinase Inhibitors in Natural Products" .PHYTOCHEMICAL ANALYSIS 28,2(2017):115-124.
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