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Simultaneous determination of senecionine, adonifoline and their metabolites in rat serum by UPLC-ESIMS and its application in pharmacokinetic studies 期刊论文

编号：

9c1256c2-79fa-4990-b525-fc8c6f81033b
作者：

Xiong, Aizhen(熊爱珍)#^[1,2]Li, Yan^[3];Yang, Li(杨莉)^[1,2]Gao, Jiangguo^[1,3];He, Yuqi^[1,2];Wang, Changhong(王长虹)*^[1,2]Wang, Zhengtao(王峥涛)^[1,2]
语种：

英文
期刊：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS ISSN：0731-7085 2009 年 50 卷 5 期 (1070 - 1074) ; DEC 5
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关键词：

Hepatotoxic pyrrolizidine alkaloids Senecionine Adonifoline UPLC-ESIMS Solid phase extraction Pharmacokinetics
摘要：

A rapid, selective and sensitive ultra-performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESIMS) method was firstly developed and validated for the simultaneous determination of two hepatotoxic pyrrolizidine alkaloids (HPAs), senecionine (SEN), adonifoline (ADO), and their N-oxides (SENNOX and ADONOX), the main metabolites in rat serum. The whole analysis was achieved within 4.5 min by gradient elution on an ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, i.d. 1.7 mu m) following a solid phase extraction for serum samples. Good linearity was achieved using weighted (1/x(2)) least squares linear regression over a 1600-fold dynamic range for SEN and ADO (LLOQ was about 0.006 mu g/ml) and 800-fold dynamic range for SENNOX and ADONOX (LLOQ was about 0.012 mu g/ml). The R.S.D. of intra- and inter-day precision was below 4.91% and 11.15% respectively, while the R.E. of accuracy was within 4.52%, 6.81%, 2.69%, and 7.12% for SEN, SENNOX, ADO, and ADONOX, respectively. The developed method was successfully applied to the in vivo pharmacokinetic study in rats after intravenous administration of SEN and ADO. (C) 2009 Elsevier B.V. All rights reserved.
推荐引用方式
GB/T 7714：

Xiong Aizhen,Li Yan,Yang Li, et al. Simultaneous determination of senecionine, adonifoline and their metabolites in rat serum by UPLC-ESIMS and its application in pharmacokinetic studies [J].JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2009,50(5):1070-1074.
APA：

Xiong Aizhen,Li Yan,Yang Li,Gao Jiangguo,&Wang Zhengtao.(2009).Simultaneous determination of senecionine, adonifoline and their metabolites in rat serum by UPLC-ESIMS and its application in pharmacokinetic studies .JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,50(5):1070-1074.
MLA：

Xiong Aizhen, et al. "Simultaneous determination of senecionine, adonifoline and their metabolites in rat serum by UPLC-ESIMS and its application in pharmacokinetic studies" .JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 50,5(2009):1070-1074.
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