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Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe 期刊论文

编号：

9bedd1ce-7a37-499e-bb51-483b53f7bb9f
作者：

Jin, Qiang#^[1,2,3,5]Feng, Lei#^[4,6]Zhang, ShuiJun^[1,5];Wang, DanDan^[2]Wang, FangJun^[3];Zhang, Yi^[1];Cui, JingNan^[4];Guo, WenZhi^[1];Ge, GuangBo*^[2,3]Yang, Ling*^[2]
语种：

英文
期刊：

ANALYTICAL CHEMISTRY ISSN：0003-2700 2017 年 89 卷 18 期 (9884 - 9891) ; SEP 19
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摘要：

In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases.
推荐引用方式
GB/T 7714：

Jin Qiang,Feng Lei,Zhang Shui-Jun, et al. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe [J].ANALYTICAL CHEMISTRY,2017,89(18):9884-9891.
APA：

Jin Qiang,Feng Lei,Zhang Shui-Jun,Wang Dan-Dan,&Yang Ling.(2017).Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe .ANALYTICAL CHEMISTRY,89(18):9884-9891.
MLA：

Jin Qiang, et al. "Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe" .ANALYTICAL CHEMISTRY 89,18(2017):9884-9891.
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