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The expression of osteoprotegerin is required for maintaining the intervertebral disc endplate of aged mice 期刊论文

编号：

976604b8-b3ff-4df7-9314-a314a32581f6
作者：

Liang, QianQian(梁倩倩)#^[1,2]Li, XiaoFeng(李晓峰)#^[1,2]Zhou, Quan(周泉)^[1,2]Xing, Lianping^[3];Cheng, ShaoDan^[1,2];Ding, DaoFang^[1,2];Xu, LeQin^[1,2];Tang, DeZhi(唐德志)^[1,2]Bian, Qin(卞琴)^[1,2]Xi, ZhiJie^[1,2];Zhou, Chongjian^[1,2];Shi, Qi(施杞)^[1,2]Wang, YongJun(王拥军)*^[1,2]
语种：

英文
期刊：

BONE ISSN：8756-3282 2011 年 48 卷 6 期 (1362 - 1369) ; JUN
收录：
关键词：

Osteoprotegerin Intervertebral disc Cartilage endplate Osteoclast Intervertebral disc degeneration
摘要：

Objective: Human chondrocytes and annulus fibrosus cells of intervertebral disc (IVD) express osteoprotegerin (OPG), but the effect of OPG on the pathogenesis of IVD degeneration remains unknown. Here we assessed the phenotype change of IVD in OPG(-/-) mice.
Methods: The IVDs from 12-, 20-, and 28-week-old OPG(-/-) mice and WT controls were subjected to histologic analyses including TRAP staining for osteoclasts, immunostaining for OPG and type I collagen protein expression, and TUNEL staining for apoptosis. The IVD tissues were also subjected to real time RT-PCR for mRNA expression of genes for osteoblast-osterix, ALP, and osteocalcin; for osteoclasts-trap, rank, mmp9 and cathepsin K, and for chondrocytes-aggrecan, mmp13 and Col10.
Results: OPG protein expresses at the cells of endplate cartilage and annulus fibrosis in IVDs of WT mice. Compared to WT mice, OPG(-/-) mice developed aging related cartilage loss and bony tissue appearance at the endplate. Stating from 20 weeks of age, IVDs from OPG(-/-) mice expressed significantly increased mmp13 and Col10 levels, which is associated with increased osteoblast number and elevated expression of osteoblast marker genes. Furthermore, TRAP+ osteoclasts were presented in the endplate cartilage of OPG(-/-) mice. These osteoclasts localized adjacently to and erosion into the cartilage. Increased expression of RANK, mmp9 and cathepsin k was detected in OPG(-/-) IVDs.
Conclusions: OPG at IVD plays an important role for maintaining the integrity of endplate cartilage during aging by preventing endplate cartilage from osteoclast-mediated resorption. (C) 2011 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Liang Qian-Qian,Li Xiao-Feng,Zhou Quan, et al. The expression of osteoprotegerin is required for maintaining the intervertebral disc endplate of aged mice [J].BONE,2011,48(6):1362-1369.
APA：

Liang Qian-Qian,Li Xiao-Feng,Zhou Quan,Xing Lianping,&Wang Yong-Jun.(2011).The expression of osteoprotegerin is required for maintaining the intervertebral disc endplate of aged mice .BONE,48(6):1362-1369.
MLA：

Liang Qian-Qian, et al. "The expression of osteoprotegerin is required for maintaining the intervertebral disc endplate of aged mice" .BONE 48,6(2011):1362-1369.
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