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Alterations in microRNA expression profiles in inflamed and noninflamed ascending colon mucosae of patients with active Crohn's disease 期刊论文

编号：

93858b3a-d086-403a-9d18-f52a34d5bfd1
作者：

Wu, Lu Yi(吴璐一)#^[1,2]Ma, Xiao Peng(马晓芃)^[2]Shi, Yin(施茵)^[2]Bao, Chun Hui(包春辉)^[2]Jin, Xiao Ming^[5];Lu, Yuan^[3];Zhao, Ji Meng^[2];Zhou, Ci Li^[2];Chen, Dai^[4];Liu, Hui Rong(刘慧荣)*^[2]
语种：

英文
期刊：

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY ISSN：0815-9319 2017 年 32 卷 10 期 (1706 - 1715) ; OCT
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关键词：

ascending colon mucosa Crohn's disease microRNA expression profiles mRNAs
摘要：

Background and Aim: The microRNA (miRNA) expression profiles of the terminal ileum, sigmoid colon, and rectal mucosa of adult patients with active Crohn's disease (CD) have been previously reported. The purpose of this study was to identify dysregulated miRNAs in the mucosa of the ascending colon.
Methods: Biopsy tissue samples were taken from the mucosae of inflammatory (iCD) or noninflammatory (niCD) areas of the ascending colons of adult patients with active CD. miRNA and mRNA expression profiles were detected using microarray analyses. miRNAs and messenger RNAs (mRNAs) demonstrating significant differences were validated via quantitative real-time polymerase chain reaction. Luciferase reporter genes were used to measure two miRNAs inhibition of potential target genes in human 293T cells in vitro.
Results: Compared with the healthy control group, the ascending colon miRNA expression profiles revealed that 43 miRNAs were significantly upregulated and 35 were downregulated in the iCD group. The mRNA expression profiles indicated that 3370 transcripts were significantly differentially expressed in the ascending colon, with 2169 upregulated and 1201 downregulated mRNAs in the iCD group, and only 20 miRNAs demonstrated significant differential expression in the niCD group. In contrast, nearly 100 miRNAs significantly varied between the iCD and niCD groups. Finally, luciferase reporter gene assays showed that hsa-miR-16-1 directly regulated the human C10orf54 gene and that they were negatively correlated.
Conclusions: Our results indicated that the differentially expressed miRNAs and mRNAs were related to immune inflammation and intestinal flora. The data provide preliminary evidence that the occurrence of CD involves the inhibition of C10orf54 expression by hsa-miR-16-1.
推荐引用方式
GB/T 7714：

Wu Lu Yi,Ma Xiao Peng,Shi Yin, et al. Alterations in microRNA expression profiles in inflamed and noninflamed ascending colon mucosae of patients with active Crohn's disease [J].JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2017,32(10):1706-1715.
APA：

Wu Lu Yi,Ma Xiao Peng,Shi Yin,Bao Chun Hui,&Liu Hui Rong.(2017).Alterations in microRNA expression profiles in inflamed and noninflamed ascending colon mucosae of patients with active Crohn's disease .JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,32(10):1706-1715.
MLA：

Wu Lu Yi, et al. "Alterations in microRNA expression profiles in inflamed and noninflamed ascending colon mucosae of patients with active Crohn's disease" .JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 32,10(2017):1706-1715.
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