To improve the efficacy of salmon calcitonin (SCT) on disuse osteoporosis-induced bone fracture, the sustained-release vehicles delivered to bone fractures should be developed. In this study, SCT-loaded poly(d,l-lactic-co-glycolic acid) microspheres (SCT-PLGA MS) with 20 kDa-COOH and 40 kDa-COOH (SCT-PLGA-20 COOH MS and SCT-PLGA-40 COOH MS) are incorporated into calcium phosphate cement (CPC) at various mass ratios. The cumulative release and mechanical properties of SCT-PLGA MS/CPC composites decrease as PLGA MS/CPC mass ratio increase. Scanning electron microscopy images and the mass loss of composites indicate the MS from 20% SCT-PLGA MS/CPC composites have mostly degraded after 8 weeks. In addition, SCT released from the 20% SCT-PLGA-20 MS/CPC composites significantly facilitate proliferation and differentiation of MC3T3-E1 cells. In conclusion, 20% SCT-PLGA-20 COOH MS/CPC composites exhibit the sustained drug release, proper CPC degradation, good mechanical properties, and preferable osteoblast proliferation, which would be beneficial to their in vivo applications. (c) 2017 Wiley Periodicals, Inc.
[1]Fudan Univ, Sch Life Sci, Dept Pharmaceut Sci, Shanghai 200438, Peoples R China.
[2]Tongji Univ, Shanghai East Hosp, Sch Med, Dept Spine, Shanghai 200120, Peoples R China.
[3]Shanghai Tradit Med Univ, Putuo Hosp, Orthopaed Dept, Shanghai 200062, Peoples R China.
(8.0+极速模式)