How oxidative stress contributes to neuro-inflammation and chronic pain is documented, and methane is reported to protect against ischemia-reperfusion injury in the nervous system via anti-inflammatory and antioxidant properties. We studied whether methane in the form of methane rich saline (MS) has analgesic effects in a monoarthritis (MA) rat model of chronic inflammatory pain. Single and repeated injections of MS (i.p.) reduced MA-induced mechanical allodynia and multiple methane treatments blocked activation of glial cells, decreased IL-1 beta and TNF-alpha production and MMP-2 activity, and upregulated IL-10 expression in the spinal cord on day 10 post-MA. Furthermore, MS reduced infiltrating T cells and expression of IFN-gamma and suppressed MA-induced oxidative stress (MDA and 8-OHDG), and increased superoxide dismutase and catalase activity. Thus, MS may offer anti-inflammatory and antioxidant effects to reduce chronic inflammatory pain.
[1]Second Mil Med Univ, Changhai Hosp, Dept Anesthesiol, Shanghai 200433, Peoples R China.
[2]PLA, Dept Anesthesiol, Hosp 188, Chaozhou 521000, Peoples R China.
[3]Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Anesthesiol, Shanghai 201203, Peoples R China.
[4]Zhejiang Univ, Affiliated Hosp 1, Dept Anesthesiol, Hangzhou 310006, Zhejiang, Peoples R China.
[5]Fudan Univ, Dept Anesthesiol, Shanghai Canc Ctr, Shanghai 200032, Peoples R China.
(8.0+极速模式)