The aim of the study was to investigate the effect of icariin (ICA) on cardiac aging through its effects on the SIRT6 enzyme and on the NF-kappa B pathway. Investigating the effect of ICA on the enzymatic activity of histone deacetylase SIRT6 revealed a concentration of 10(-8) mol/L ICA had a maximum activating effect on histone deacetylase SIRT6 enzymatic activity. Western analysis showed that ICA upregulated SIRT6 protein expression and downregulated NF-kappa B (p65) protein expression in animal tissues and cell models. ICA upregulated the expression of SIRT6 and had an inhibitory effect on NF-kappa B inflammatory signaling pathways as shown by decreasing mRNA levels of the NF-kappa B downstream target genes TNF-alpha, ICAM-1, IL-2, and IL-6. Those effects were mediated directly or indirectly by SIRT6. We provided evidence that inflammaging may involve a novel link between the effects of ICA on SIRT6 (a regulator of aging) and NF-kappa B (a regulator of inflammation).
[1]Shanghai Guanghua Hosp Integrat Tradit Chinese &, Ctr Dis Prevent & Treatment, Shanghai 200052, Peoples R China.
[2]Fudan Univ, Huadong Hosp, Shanghai Inst Geriatr, Dept Geriatr, Shanghai 200040, Peoples R China.
[3]Pregnancy Res Ctr, Dept Perinatal Med, Parkville, Vic 3052, Australia.
[4]Univ Melbourne, Royal Hosp Women, Dept Obstet & Gynaecol, Parkville, Vic 3052, Australia.
[5]China Three Gorges Univ, Coll Med, Yichang 443002, Peoples R China.
[6]Fudan Univ, Huashan Hosp, Key Lab Cellular & Mol Biol, Shanghai 200040, Peoples R China.
[7]Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Dis Prevent & Healthcare, Shanghai 200032, Peoples R China.
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