Aims: Curcumin can suppress human prostate cancer (HuPCa) cell proliferation and invasion. However, it is not known whether curcumin can inhibit HuPCa stem cell (HuPCaSC) proliferation and invasion. Materials and Methods: We used methyl thiazolyl tetrazolium and Transwell assays to examine the proliferation and invasion of the HuPCaSC lines DU145 and 22Rv1 following curcumin or dimethyl sulfoxide (control) treatment. The microRNA (miRNA) expression levels in the DLK1-DIO3 imprinted genomic region in the cells and in tumor tissues from patients with PCa were examined using microarray and quantitative PCR. Results: The median inhibitory concentration of curcumin significantly inhibited HuPCaSC proliferation and invasion in vitro. The miR-770-5p and miR-1247 expression levels in the DLK1-DIO3 imprinted gene cluster were significantly different between the curcumin-treated and control HuPCaSCs. Overexpression of these positive miRNAs significantly increased the inhibition rates of miR-770-5p- and miR-1247-transfected HuPCaSCs compared to the control miR-Mut-transfected HuPCaSCs. Last, low-tumor grade PCa tissues had higher miR-770-5p and miR-1247 expression levels than high-grade tumor tissues. Conclusions: Curcumin can suppress HuPCaSC proliferation and invasion in vitro by modulating specific miRNAs in the DLK1-DIO3 imprinted gene cluster.
[1]Shanghai Univ Tradit Chinese Med, Shanghai Geriatr Inst Chinese Med, Longhua Hosp, Shanghai 200031, Peoples R China.
[2]Capital Med Univ, Beijing Friendship Hosp, Dept Urol, Beijing, Peoples R China.
[3]Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Shanghai, Peoples R China.
[4]Yale Univ, Sch Med, Dept Pathol, 10 Amistad St, New Haven, CT 06510 USA.
(8.0+极速模式)