1.Pinoresinol di-O--d-glucopyranoside (PDG), geniposide (GE), geniposidic acid (GA), aucubin (AN) and chlorogenic acid (CA) are the representative active ingredients in Eucommiae cortex (EC), which may be estrogenic.2.The ultra high-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of the five ingredients showed good linearity, low limits of quantification and high extraction recoveries, as well as acceptable precision, accuracy and stability in mice plasma and tissue samples (liver, spleen, kidney and uterus). It was successfully applied to the comparative study on pharmacokinetics and tissue distribution of PDG, GE, GA, AN and CA between normal and ovariectomized (OVX) mice.3.The results indicated that except CA, the plasma and tissue concentrations of PDG, GE, GA in OVX mice were all greater than those in normal mice. AN could only be detected in the plasma and liver homogenate of normal mice, which was poorly absorbed in OVX mice and low in other measured tissues. PDG, GE and GA seem to be better absorbed in OVX mice than in normal mice proved by the remarkable increased value of AUC(0-) and C-max. It is beneficial that PDG, GE, GA have better plasma absorption and tissue distribution in pathological state.
[1]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, MOE Key Lab Standardizat Chinese Med, Shanghai 201210, Peoples R China.
[3]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Key Lab Pharmacol Compound Chinese Med Shanghai, Shanghai 201210, Peoples R China.
[4]Xi An Jiao Tong Univ, Coll Med, Hong Hui Hosp, Xian 710049, Peoples R China.
[5]Shanghai Univ Tradit Chinese Med, Res Ctr Tradit Chinese Med Complex Syst, Shanghai 201210, Peoples R China.
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