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Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells 期刊论文

编号：

791c3f96-5c0e-4cd0-b977-65b6471533e7
作者：

Wu, Qing#*^[1]Chen, YongFeng#^[2]Fu, Jie#^[3]You, QiHan^[4];Wang, ShouMei(王守梅)^[5]Huang, Xue^[5];Feng, XiaoJun^[5];Zhang, ShuHui(张树辉)*^[5]
语种：

英文
期刊：

CELLULAR ONCOLOGY ISSN：2211-3428 2014 年 37 卷 6 期 (399 - 407) ; DEC
收录：
关键词：

Centromere protein A Lung adenocarcinoma Cell cycle regulation Gene silencing
摘要：

Deregulation of centromere protein (CENP)-A, a centromere-specific histone variant, has in the past been linked to cancer initiation and progression. Additionally, our previous work has shown that CENP-A upregulation predicts a poor overall survival in patients with lung adenocarcinoma. The aim of this study was to uncover the biological role of CENP-A in lung adenocarcinoma growth and invasion, including its underlying molecular mechanisms.
CENP-A expression was knocked down in human lung adenocarcinoma A549 and PC-9 cells using a short hairpin RNA (shRNA) technology. Subsequently, the effects of this knock down on the proliferation, apoptosis, cell cycle progression, colony formation, migration, invasion and tumorigenicity were assessed. Additionally, Western blot analyses were performed to examine concomitant expression changes in key proteins involved in cell cycle regulation and apoptosis.
We found that shRNA-mediated knock down of CENP-A significantly inhibited the in vitro proliferation and colony formation of A549 and PC-9 cells as compared to control shRNA-transfected cells. In addition, CENP-A down-regulation was found to induce G0/G1 cell cycle arrest and apoptosis, and to inhibit the in vitro migration and invasion of A549 and PC-9 cells. Down-regulation of CENP-A was also found to significantly suppress the in vivo growth of xenografted A549 cells. At the protein level, we found that the expression of p21, p27, CHK2 and Bax was markedly increased and that the expression of CCNG1, Skp2, Cks1 and Bcl-2 was markedly decreased in CENP-A down-regulated cells.
Based on our results we conclude that down-regulation of CENP-A may attenuate the aggressive phenotype of lung adenocarcinoma cells. As such, CENP-A may serve as a promising therapeutic target for lung adenocarcinoma.
推荐引用方式
GB/T 7714：

Wu Qing,Chen Yong-Feng,Fu Jie, et al. Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells [J].CELLULAR ONCOLOGY,2014,37(6):399-407.
APA：

Wu Qing,Chen Yong-Feng,Fu Jie,You Qi-Han,&Zhang Shu-Hui.(2014).Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells .CELLULAR ONCOLOGY,37(6):399-407.
MLA：

Wu Qing, et al. "Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells" .CELLULAR ONCOLOGY 37,6(2014):399-407.
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