[1]Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PRC.;State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PRC.;Key Laboratory of Theory and Therapy of Muscles and Bones of Ministry of Education, Shanghai, PRC.
[2]Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PRC.
[3]School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, PRC.
[4]Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PRC.;Key Laboratory of Theory and Therapy of Muscles and Bones of Ministry of Education, Shanghai, PRC.
[5]Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, PRC.
[6]Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, PRC.;Key Laboratory of Theory and Therapy of Muscles and Bones of Ministry of Education, Shanghai, PRC.;School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, PRC.
[7]State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PRC.;Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PRC.
Our previous study reported that the in vitro osteogenic effects of 8-prenylgenistein (8PG) were more potent than its parent compound genistein. This study aimed to evaluate the osteoprotective effects of 8PG in ovariectomized (OVX) mice as well as to characterize its estrogenic effects in uterus. Mature OVX mice were treated with phytoestrogen-free diet containing 8PG or genistein. Trabecular bone mass and most of the micro-structural parameters were ameliorated at the distal femoral metaphysis in OVX mice upon treatment with genistein and both doses of 8PG. The beneficial effects of 8PG on trabecular bone were confirmed by safranin O and ABHO staining. 8PG markedly inhibited the ovariectomy-induced mRNA expressions of RANKL/OPG, ALP, COL, OCN, cathepsin K and ER-α in bone. In contrast, genistein further increased the ovariectomy-induced ER-α expression in bone. The uterus index was increased in genistein-treated group. Genistein up-regulated the expression of ER-α and PR, while 8PG significantly down-regulated the ER-α and C3 expression in uterus of OVX mice. Moreover, genistein, but not 8PG, increased expressions of ER-α, PCNA and C3 in Ishikawa cell. This study suggested that 8PG improved trabecular bone properties in OVX mice without exerting uterotrophic effects and its estrogenic actions were distinct from those of genistein.;
(8.0+极速模式)