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Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China Between 2010 and 2015 期刊论文

编号：

752a50b7-158c-432f-a0bb-9af6023f2f68
作者：

Zhang, WenXia(张雯霞)#^[1]Chen, HongYou^[2];Tu, LiHong(屠丽红)^[2]Xi, ManFang^[2];Chen, Min*^[2]Zhang, Jue(张珏)*^[1]
语种：

英文
期刊：

MICROBIAL DRUG RESISTANCE ISSN：1076-6294 2019 年 25 卷 2 期 (212 - 218) ; MAR 1
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关键词：

Shigella antimicrobial resistance qnrS quinolone DNA gyrase topoisomerase IV
摘要：

Objectives: This study aimed to investigate the antimicrobial susceptibility of Shigella isolated in Shanghai, China and to determine the genetic basis of its resistance to fluoroquinolones.
Materials and Methods: A total of 402 strains of Shigella, including 145 Shigella flexneri and 257 Shigella sonnei isolates, were analyzed. The Kirby-Bauer disk diffusion method was used to determine the susceptibility of the strains to 13 antimicrobials. Minimum inhibitory concentration of ciprofloxacin was determined by E-test. Mutations within the quinolone resistance-determining regions (QRDRs) of gyrA and parC and in the plasmid-mediated quinolone resistance (PMQR) genes, including qnrA, qnrB, qnrS, and aac (6')-Ib-cr, were detected by polymerase chain reaction. All the products were then sequenced.
Results: Most of the Shigella isolates were found to be resistant to nalidixic acid (96.4%), streptomycin (96.4%), ampicillin (86.2%), tetracycline (79.8%), and sulfamethoxazole/trimethoprim (80.6%). S. flexneri isolates showed a significantly higher resistance to cefepime (33.6%), ciprofloxacin (54.2%), norfloxacin (34.1%), and levofloxacin (12.1%) compared with that observed for the S. sonnei strains (chi(2) analysis, p<0.05). Three mutations (Ser83, Asp87, and His211) in gyrA and one mutation (Ser80) in parC were detected. Of 257 S. sonnei isolates, 11.7% possessed gyrA mutations and 2% had parC mutations. Of 145 S. flexneri isolates, 98.6% possessed gyrA mutations and 97.9% had parC mutations. The plasmid-mediated resistance genes of qnrS and aac (6)-Ib-cr were detected among 17 strains (4.2%).
Conclusions: The mutation percentage within the QRDR of S. flexneri was as high as 98.6 in gyrA and 97.9 in parC. The significant abundance of mutations within QRDRs conferred high levels of fluoroquinolone resistance. Moreover, the PMQR genes, particularly qnrS, played an important role in the decreased susceptibility of Shigella to fluoroquinolones.
推荐引用方式
GB/T 7714：

Zhang Wen-Xia,Chen Hong-You,Tu Li-Hong, et al. Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China Between 2010 and 2015 [J].MICROBIAL DRUG RESISTANCE,2019,25(2):212-218.
APA：

Zhang Wen-Xia,Chen Hong-You,Tu Li-Hong,Xi Man-Fang,&Zhang Jue.(2019).Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China Between 2010 and 2015 .MICROBIAL DRUG RESISTANCE,25(2):212-218.
MLA：

Zhang Wen-Xia, et al. "Fluoroquinolone Resistance Mechanisms in Shigella Isolates in Shanghai, China Between 2010 and 2015" .MICROBIAL DRUG RESISTANCE 25,2(2019):212-218.
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