This study evaluates the chemical structure of a heteropolysaccharide (PSF-W-1) from the endophytic fungus Trichoderma atroviride and its effects on the production of tanshinones in Salvia miltiorrhiza hairy roots. The total carbohydrate content of isolated PSF-W-1 was determined to be 97.72%. PSF-W-1 has a relative molecular weight of 36.13 kDa and contains mannose, glucose and galactose in molar ratios of 1.00: 4.86: 2.25. Through methylation analysis, IR and NMR, PSF-W-1 was determined to possess a backbone of -> 4)-beta-D-Glcp-(1 -> 6)-alpha-D-Galp-(1 -> 4)-beta-D-Manp-(1 -> 6)-alpha-D-Galp-(1 -> with two side chains beta-D-Glcp-(1 -> 4)-beta-D-Glcp-(1 -> attached to O3 of 1,6-alpha-D-Galp. Bioactivity tests suggested that PSF-W-1 was responsible for boosting the S. miltiorrhiza hairy root growth and the biosynthesis of dihydrotanshinone I, tanshinone I, tanshinone IIA and cryptotanshinone in hairy roots. According to this study, PSF-W-1 might be utilized as a potent stimulator of tanshinones synthesis.
[1]Zhejiang Chinese Med Univ, Sch Pharm, Hangzhou 310053, Zhejiang, Peoples R China.
[2]Army Med Univ, Sch Pharm, Dept Pharmacognosy, Chongqing 400038, Peoples R China.
[3]Navy Med Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 200433, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China.
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