Eight previously undescribed oleanane-type triterpenoid saponins, clematangoticosides A-H, together with eight known saponins, were isolated from the whole plants of Clematis tangutica (Maxim.) Korsh. Their structures were elucidated by extensive spectroscopic analysis, in combination with chemical methods (acid hydrolysis and mild alkaline hydrolysis). Clematangoticosides D-G were found to be unusual 23, 28-bidesmosidic glycosides. The cytotoxic activities of all of the isolated saponins were evaluated against the four human cancer cell lines SGC-7901, HepG2, HL-60 and U251MG. Clematoside S, sapindoside B, kalopanax saponin A, and koelreuteria saponin A exhibited cytotoxicity against all of the test cancer cell lines with 1050 values in the range of 1.88-27.20 mu M, while clematangoticoside D and F showed selective cytotoxicity against SGC-7901 with IC50 values of 24.22 and 2135 mu M, respectively. (C) 2016 Elsevier Ltd. All rights reserved.
[1]Chinese Acad Sci, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu Inst Biol, Chengdu 610041, Peoples R China.
[2]Tibet Autonomous Reg Inst Food & Drug Control, Lhasa 850000, Peoples R China.
[3]Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China.
[4]Syngenta Jealotts Hill Int Res Ctr, Bracknell RG42 6EY, Berks, England.
(8.0+极速模式)