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MS) 期刊论文

编号：

63c5b827-0d7e-450d-90c3-5159d4cc22cb
作者：

Zhu, Yunyun(朱云云)#^[1]Cong, Wenjuan(从文娟)*^[1]Shen, Lan(沈岚)^[1,2]Wei, Hai(魏海)^[3]Wang, Yuan^[1];Wang, Lingyi^[2];Ruan, Kefeng(阮克锋)^[1]Wu, Fei(吴飞)^[1]Feng, Yi(冯怡)^[1]
语种：

英文
期刊：

MOLECULAR BIOSYSTEMS ISSN：1742-206X 2014 年 10 卷 2 期 (304 - 312)
收录：
摘要：

Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder with systemic complications and has been a worldwide epidemic. Ophiopogon japonicus is a traditional Chinese medicine used to treat diabetes for thousands of years. From our previous work, we know that MDG-1, a water-soluble beta-D-fructan polysaccharide from O. japonicas could treat T2DM experimentally. However, MDG-1 is poorly absorbed and its mechanism of action is still unknown. Therefore, a GC TOF/MS-based metabonomic approach in combination with multivariate statistical analysis was performed to investigate the mechanism of MDG-1 in a spontaneous diabetic model. Female diabetic KKay mice (21 weeks old) were randomly divided into a diabetic group (n = 6, gavaged with distilled water) and a MDG-1-Diabetic group (n = 7, gavaged with MDG-1, 300 mg kg(-1)) and female C57BL/6 mice (21 weeks old) were set as controls (n = 6, gavaged with distilled water). After 8-weeks of treatment, feces samples were collected for GC-TOF/MS analysis. Consequently, 12 potential biomarkers were identified, including monosugars (D-tagatose, D-lyxose, D-erythrose, xylo-hexos-5-ulose, 2-deoxy-galactose), butanedioic acid, amino acids (phenylalanine, L-lysine, L-methionine, L-aspartic acid) and purine derivatives (7H-purine, 2'-deoxyinosine). We assume the monosugars and butanedioic acid were the fermentation products of MDG-1 by intestinal microbes and MDG-1 actions against diabetes might be accomplished through the absorbable monosugars and butanedioic acid via suppressing intestinal glucose absorption, enhancing liver glycogenesis, inhibiting glycogenolysis and promoting GLP-1 secretion. Besides, MDG-1 might alleviate diabetes and diabetic nephropathy by reducing 7H-purine and 2'-deoxyinosine. Further omics-driven studies including genomics, proteomics and metabonomics were considered to be carried out to provide direct evidence of gut microbiome contribution to MDG-1 actions.
推荐引用方式
GB/T 7714：

Zhu Yunyun,Cong Wenjuan,Shen Lan, et al. Fecal metabonomic study of a polysaccharide, MDG-1 from Ophiopogon japonicus on diabetic mice based on gas chromatography/time-of-flight mass spectrometry (GC TOF/MS) [J].MOLECULAR BIOSYSTEMS,2014,10(2):304-312.
APA：

Zhu Yunyun,Cong Wenjuan,Shen Lan,Wei Hai,&Feng Yi.(2014).Fecal metabonomic study of a polysaccharide, MDG-1 from Ophiopogon japonicus on diabetic mice based on gas chromatography/time-of-flight mass spectrometry (GC TOF/MS) .MOLECULAR BIOSYSTEMS,10(2):304-312.
MLA：

Zhu Yunyun, et al. "Fecal metabonomic study of a polysaccharide, MDG-1 from Ophiopogon japonicus on diabetic mice based on gas chromatography/time-of-flight mass spectrometry (GC TOF/MS)" .MOLECULAR BIOSYSTEMS 10,2(2014):304-312.
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