Background and aim: Silver has been widely used as a topical antimicrobial agent in burn wound care. In a previous study, we demonstrated the introduction of nano-silver particles to porcine small intestinal submucosa (NS-PSIS) led to significant enhancement in antibacterial property in repairing contaminated abdominal defect. In this study, we explored the efficacy of NS-PSIS in the treatment of Pseudomonas aeruginosa-infected partial-thickness burn wounds.
Methods: 48 male Sprague-Dawley rats were divided into four groups of equal number. Standardized and reproducible Pseudomonas aeruginosa-infected partial-thickness thermal burns wound model were created using these rats. NS-PSIS, PSIS (porcine small intestinal submucosa) or lipido-colloid dressingss (Urgotul (TM)) were tested for 14 days to assess their ability to heal the rats' burn wounds. Control group was without any treatment after the establishment of infected burn-wound. The wound contraction rate, animal body weight change, histological examination, and the quantification of IL-6 and C-reactive protein (CRP) were measured to evaluate the healing effects.
Results: NS-PSIS significantly promoted wound healing and recovered the normal growth of rats. There were significantly lower expression levels of pro-inflammatory cytokine (IL-6) and CRP in NS-PSIS group as compared with the PSIS or Urgotul group in the treatment of infected partial-thickness burn wounds. Histological exams revealed significant less inflammatory cells infiltrating, more re-epithelization and neovascularization in NS-PSIS group. There were also less inflammatory cells infiltrations in the major organs in NS-PSIS group.
Conclusions: Nano-silver modified porcine small intestinal submucosa (NS-PSIS) can be used as a biological derivative dressing for the treatment of infected partial-thickness burn wounds. (C) 2018 Elsevier Ltd and ISBI. All rights reserved.
[1]Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Gastrointestinal Surg, 528 Zhangheng Rd, Shanghai, Peoples R China.
[2]Shanghai Baoshan Dist Combining Tradit Chinese &, Dept Surg, 181 Youyi Rd, Shanghai, Peoples R China.
[3]Second Mil Med Univ, Changzheng Hosp, Div Colorectal Surg, 415 Fengyang Rd, Shanghai, Peoples R China.
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