首页 / 院系成果 / 成果详情页

Smad7 Promotes Healing of Radiotherapy-Induced Oral Mucositis without Compromising Oral Cancer Therapy in a Xenograft Mouse Model 期刊论文

编号：

5f6ec617-3e05-472a-b75c-9ecf066278f1
作者：

Luo, Jingjing#^[1,2]Bian, Li#^[2,3,4]Blevins, Melanie A.^[5];Wang, Dongyan^[2,4];Liang, Chao(梁超)^[2,8]Du, Danfeng^[2,9,10];Wu, Fanglong^[1,2];Holwerda, Barry^[6];Zhao, Rui^[5];Raben, David^[7];Zhou, Hongmei*^[1]Young, Christian D.*^[2,4]Wang, XiaoJing*^[2,4]
语种：

英文
期刊：

CLINICAL CANCER RESEARCH ISSN：1078-0432 2019 年 25 卷 2 期 (808 - 818) ; JAN 15
收录：
摘要：

Purpose: We previously reported preventive and therapeutic effects of Smad7, a multifunctional protein, on radiotherapy (RT)-induced mucositis in mice without promoting human oral cancer cell survival or migration in vitro. The current study aims to determine whether a Smad7-based biologic can treat existing oral mucositis during radiotherapy for oral cancer and whether this treatment compromises RT-induced cancer cell killing in neighboring oral cancer.
Experimental Design: We transplanted human oral cancer cells into the tongues of mice and applied craniofacial irradiation to simultaneously kill tumor cells and induce oral mucositis, thus modeling RT and mucositis in oral cancer patients. We topically applied a recombinant human Smad7 protein fused with the cell-penetrating Tat tag (Tat-Smad7) to the oral mucosa of tumor-bearing mice post RT when oral mucositis began to develop.
Results: Topically applied Tat-Smad7 penetrated cells in both the oral mucosa and oral cancer, attenuating TGF beta and NF-kappa B signaling as well as inflammation at both sites. Tat-Smad7 treatment alleviated oral mucositis with reductions in DNA damage and apoptosis in keratinocytes, but increased keratinocyte proliferation compared with vehicle-treated mucositis lesions. In contrast, adjacent oral cancer exposed to Tat-Smad7 did not show alterations in proliferation or direct DNA damage, but showed increased oxidative stress damage and apoptosis compared with tumors treated with vehicle.
Conclusions: Our results suggest that short-course Tat-Smad7 application to oral mucositis promotes its healing but does not compromise the cytotoxic effect of RT on oral cancer and has context-specific effects on oral mucosa versus oral cancer.
推荐引用方式
GB/T 7714：

Luo Jingjing,Bian Li,Blevins Melanie A., et al. Smad7 Promotes Healing of Radiotherapy-Induced Oral Mucositis without Compromising Oral Cancer Therapy in a Xenograft Mouse Model [J].CLINICAL CANCER RESEARCH,2019,25(2):808-818.
APA：

Luo Jingjing,Bian Li,Blevins Melanie A.,Wang Dongyan,&Wang Xiao-Jing.(2019).Smad7 Promotes Healing of Radiotherapy-Induced Oral Mucositis without Compromising Oral Cancer Therapy in a Xenograft Mouse Model .CLINICAL CANCER RESEARCH,25(2):808-818.
MLA：

Luo Jingjing, et al. "Smad7 Promotes Healing of Radiotherapy-Induced Oral Mucositis without Compromising Oral Cancer Therapy in a Xenograft Mouse Model" .CLINICAL CANCER RESEARCH 25,2(2019):808-818.
入库时间：

1/6/2020 6:09:06 PM
更新时间：

1/6/2020 6:09:06 PM
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：106  下载次数：0

分享到：

浏览次数：106

下载次数：0

打印次数：0