Circulating tumour cells isolated from the peripheral blood of patients have the advantage of being non-invasive. The aim of this study was to develop an effective method for detecting pancreatic cancer micrometastasis from peripheral blood and explore the clinical application value of the separation of circulating pancreatic cancer cells using epithelial cell adhesion molecule (EpCAM) lipid magnetic spheres (ELMS) and plastin monoclonal antibody for identification. The pancreatic cancer PANC-1 cells were respectively added to phosphate-buffered saline (PBS) and fresh blood provided by healthy volunteers to detect cell recovery using ELMS. Moreover, trials were conducted using nude mice with tumour and samples from patients with pancreatic cancer to evaluate the effect of CTC isolation and identification. The prepared ELMS captured PANC-1 cells from blood with high efficiency, which was similar to the efficiency obtained with nude mice with tumour and the samples from patients with pancreatic cancer, in agreement with the clinical test results. This study is based on the high efficiency of the EpCAM magnetic nanolipid sphere separation system capturing CTC of pancreatic cancer, which provides a strong support for investigating pancreatic cancer, in terms of its early diagnosis, preoperative and post-operative analysis and analysing the treatment effects.
[1]Cent S Univ, Xiangya Hosp, Key Lab Nanobiol Technol, Changsha 410008, Hunan, Peoples R China.
[2]Shanghai Univ Tradit Chinese Med, Putuo Hosp, Shanghai 200062, Peoples R China.
[3]Shanghai Jiao Tong Univ, Sch Med, State Key Lab Oncogenes & Related Genes, Shanghai Canc Inst,Renji Hosp, Shanghai 200032, Peoples R China.
(8.0+极速模式)