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Characterization of triptolide hydroxylation by cytochrome P450 in human and rat liver microsomes 期刊论文

编号：

5ca96e4a-e03b-4238-b2c4-32f02f1b4476
作者：

Li, W.#^[1,5]Liu, Y.^[1];He, Y. Q.^[2];Zhang, J. W.^[1];Gao, Y.^[3];Ge, G. B.^[1,5];Liu, H. X.^[1,5];Huo, H.^[1];Liu, H. T.^[1,5];Wang, L. M.^[3];Sun, J.^[3];Wang, Q.^[4];Yang, L.*^[1]
语种：

英文
期刊：

XENOBIOTICA ISSN：0049-8254 2008 年 38 卷 12 期 (1551 - 1565)
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关键词：

Triptolide cytochrome P450 mono-hydroxylated metabolite
摘要：

Triptolide, the primary active component of a traditional Chinese medicine Tripterygium wilfordii Hook F, has a wide range of pharmacological activities. In the present study, the metabolism of triptolide by cytochrome P450s was investigated in human and rat liver microsomes. Triptolide was converted to four metabolites (M-1, M-2, M-3, and M-4) in rat liver microsomes and three (M-2, M-3, and M-4) in human liver microsomes. All the products were identified as mono-hydroxylated triptolides by liquid chromatography-mass spectrometry (LC-MS). The studies with chemical selective inhibitors, complementary DNA-expressed human cytochrome P450s, correlation analysis, and enzyme kinetics were also conducted. The results demonstrate that CYP3A4 and CYP2C19 could be involved in the metabolism of triptolide in human liver, and that CYP3A4 was the primary isoform responsible for its hydroxylation.
推荐引用方式
GB/T 7714：

Li W.,Liu Y.,He Y. -Q., et al. Characterization of triptolide hydroxylation by cytochrome P450 in human and rat liver microsomes [J].XENOBIOTICA,2008,38(12):1551-1565.
APA：

Li W.,Liu Y.,He Y. -Q.,Zhang J. -W.,&Yang L..(2008).Characterization of triptolide hydroxylation by cytochrome P450 in human and rat liver microsomes .XENOBIOTICA,38(12):1551-1565.
MLA：

Li W., et al. "Characterization of triptolide hydroxylation by cytochrome P450 in human and rat liver microsomes" .XENOBIOTICA 38,12(2008):1551-1565.
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