Gut microbiota is recognized as an indispensable "metabolic organ" that plays crucial roles in maintaining human health or initiating diseases. Spleen-yang-deficiency syndrome (SYDS) is a common syndrome of Traditional Chinese Medicine (TCM) clinic. It is a complex phenotype reflecting the overall changes of metabolism which are mainly caused by digestive disorders. However, little is known about the changes of gut microbiota and metabolism in patients with SYDS, as well as the crosstalk between gut microbiota and host metabolism. In the current study, an integrative metabolic and microbial profiling was performed on plasma, urine and feces from recruited SYDS and healthy individuals by using a LC-QTOFMS-based metabolomic and 16 s rRNA sequencing approaches. Our results showed a potentially significant contribution of gut dysbiosis to the metabolic disorders in SYDS. By integrating the differential gut bacteria with the metabolites, the results revealed some active bacterium of norank_f_CFT112H7, f_lachnospiraceae and bacteroides were closely involved in host mucosal integrity, bile acid metabolism and polysaccharides decomposition. Therefore, our results indicated the probable involvement of gut microbiota in mediating the metabolic changes, which warrants a further investigation on the role of gut microbiota in modulating the pathogenesis of SYDS.
[1]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200000, Peoples R China.
[2]Shanghai Municipal Hosp Tradit Chinese Med, Dept Gastroenterol, Shanghai 200000, Peoples R China.
[3]Second Mil Med Univ, Sch Pharm, Shanghai 200000, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med Sci, Ctr Chinese Med Therapy & Syst Biol, Shanghai 201203, Peoples R China.
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