首页 / 院系成果 / 成果详情页

Identification of 20(S)-protopanaxatriol metabolites in rats by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance spectroscopy 期刊论文

编号：

4d0bfc1b-4d70-4d94-97b6-7383a904166d
作者：

He, Chunyong#^[1]Zhou, Dandan^[2,3,4];Li, Jia^[1];Han, Han^[3,4,5];Ji, Guang(季光)^[2]Yang, Li(杨莉)*^[3,4,5]Wang, Zhengtao(王峥涛)^[1,3,4,5]
语种：

英文
期刊：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS ISSN：0731-7085 2014 年 88 卷 (497 - 508) ; JAN 25
收录：
关键词：

20(S)-Protopanaxatriol UPLC Q/TOF-MS NMR Metabolite identification Rats
摘要：

20(S)-Protopanaxatriol (PPT), one of the aglycones of ginsenosides, has been shown to exert cardioprotective effects against myocardial ischemic injury. However, studies on PPT metabolism have rarely been reported. This study is the first to investigate the in vivo metabolism of PPT following oral administration by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS) and nuclear magnetic resonance (NMR) spectroscopy. The structures of the metabolites were identified based on the characteristics of their MS data, MS2 data, and chromatographic retention times. A total of 22 metabolites, including 17 phase land 5 phase ll metabolites, were found and tentatively identified by comparing their mass spectrometry profiles with those of PPT. Two new monooxygenation metabolites, (205,24S)-epoxy-dammarane3,6,12,25-tetraol and (20S,24R)-epoxy-dammarane-3,6,12,25-tetraol, were chemicallly synthesized and unambiguously characterized according to the NMR spectroscopic data. The metabolic pathways of PPT were proposed accordingly for the first time. Results revealed that oxidation of (1) double bonds at (Delta((24,25))) to form 24,25-epoxides, followed by rearrangement to yield 20,24-oxide forms; and (2) vinyl-methyl at C-26/27 to form corresponding carboxylic acid were the predominant metabolic pathways. Phase II metabolic pathways were proven for the first time to consist of glucuronidation and cysteine conjugation. This study provides valuable and new information on the metabolism of PPT, which is indispensable for understanding the safety and efficacy of PPT, as well as its corresponding ginsenosides. (C) 2013 Elsevier B.V. All rights reserved.
推荐引用方式
GB/T 7714：

He Chunyong,Zhou Dandan,Li Jia, et al. Identification of 20(S)-protopanaxatriol metabolites in rats by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance spectroscopy [J].JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,88:497-508.
APA：

He Chunyong,Zhou Dandan,Li Jia,Han Han,&Wang Zhengtao.(2014).Identification of 20(S)-protopanaxatriol metabolites in rats by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance spectroscopy .JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,88:497-508.
MLA：

He Chunyong, et al. "Identification of 20(S)-protopanaxatriol metabolites in rats by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance spectroscopy" .JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 88(2014):497-508.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：43  下载次数：0

分享到：

浏览次数：43

下载次数：0

打印次数：0