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Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1 alpha and epithelial mesenchymal transition 期刊论文

编号：

44ab28d0-be73-491f-b46e-9236183cc4d7
作者：

Hou, Fenggang(侯风刚)#^[1]Li, Wen#^[1]Shi, Qi(石齐)^[1]Li, Hongjia(李泓佳)^[1]Liu, Shanshan(刘姗姗)^[1]Zong, Shaoqi^[1];Ren, Jianlin(任建琳)^[1]Chai, Jie*^[2]Xu, Jian(徐建)*^[3]
语种：

英文
期刊：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE ISSN：1472-6882 2016 年 16 卷 ; NOV 2
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关键词：

Yi Ai Fang HIF-1 alpha EMT Colorectal cancer Vasculogenic mimicry
摘要：

Background: Yi Ai Fang (YAF), a traditional Chinese medicine (TCM) formula, has been identified to have anticancer activity in our previously studies. The present study aimed to explore the potential mechanism of YAF suppression of VM on colorectal cancer (CRC) in vitro and in vivo.
Methods: Cell viability was measured by CCK-8 assay. HIF-1 alpha, E-cd(E-cadherin), Claudin-4, and VIM (Vimentin) expressions level in vitro were evaluated by Western blot or RT-PCR. In addition, Human CRC HCT-116 cells were implanted in BALB/c nude mice; mice with xenografted tumors were randomly administrated vehicle (control), 8, 16, or 32 mg/mL YAF, or 1 mg/mL fluorouracil (5-FU). HIF-1 alpha, E-cd, Claudin-4, and VIM expression in these tumors were determined by IHC.
Results: YAF effectively inhibited the growth and the formation of vasculogenic mimicry (VM) of CRC cells in a dose-dependent trend. YAF restrained the formation of vasculogenic mimicry(VM) through HIF-1 alpha/EMT pathway in CRC. YAF suppressed VM was triggered by activation of E-cd and Claudin-4, which are characteristics of endothelial cells, and inhibition of HIF-1 alpha and VIM in vitro. In vivo data showed that YAF remarkably inhibited growth of the xenografted tumors. The YAF-treated tumor samples were analyzed by IHC for levels of HIF-1 alpha/EMT related proteins HIF-1 alpha, E-cd, Claudin-4, and VIM. The results indicated that YAF significantly enhanced expression of E-cd and Claudin-4, but decreased expression of HIF-1 alpha, VIM in a dose-dependent manner.
Conclusions: In conclusion, this study provided the first direct evidence that YAF inhibited the formation of VM in human CRC, suggesting that YAF may be considered as a useful target for cancer therapy.
推荐引用方式
GB/T 7714：

Hou Fenggang,Li Wen,Shi Qi, et al. Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1 alpha and epithelial mesenchymal transition [J].BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2016,16.
APA：

Hou Fenggang,Li Wen,Shi Qi,Li Hongjia,&Xu Jian.(2016).Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1 alpha and epithelial mesenchymal transition .BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,16.
MLA：

Hou Fenggang, et al. "Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1 alpha and epithelial mesenchymal transition" .BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 16(2016).
入库时间：

11/15/2019 12:33:20 PM
更新时间：

11/15/2019 12:33:20 PM
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