首页 / 院系成果 / 成果详情页

Evaluation of specific fecal protein biochips for the diagnosis of colorectal cancer 期刊论文

编号：

416a7457-cc80-493d-8c12-5b297e9db35a
作者：

Wang, HuiPeng#^[1]Wang, YangYun^[1];Pan, Jie^[1];Cen, Rong(岑戎)^[2]Cai, YuanKun*^[1]
语种：

英文
期刊：

WORLD JOURNAL OF GASTROENTEROLOGY ISSN：1007-9327 2014 年 20 卷 5 期 (1332 - 1339) ; FEB 7
收录：
关键词：

Protein biochip Feces Colorectal cancer Fibroblast growth factor-23 Thrombopoietin
摘要：

AIM: To develop and initially test a potential fecal protein biochip for the screening of colorectal cancer (CRC).
METHODS: Fecal protein from 20 colorectal cancer patients and 20 healthy controls were extracted from all of the fecal samples and screened for proteomic differences using a Biotin label-based protein array. Candidate proteins were then verified by ELISA. Finally, we will select out the significant protein and a seven-target multiplex fecal protein biochip was generated and tested for 20 fecal samples to determine the effectiveness of the biochip on identifying CRC. And the value of the protein biochip would be discussed.
RESULTS: After tested by protein biochip of the fecal protein from 20 colorectal cancer patients and 20 healthy controls and levels of calprotectin, M2-pyruvatekinase, angiopoietin-2, fibroblast growth factor- 23 (FGF-23), proteins of the matrix metalloproteinase, thrombopoietin (TPO) and interleukin-13 (IL-13) were significantly different between CRC and healthy controls. The sensitivity of all the seven proteins combined was 0.7, specificity was 0.4, and area under the receiver operating characteristics was 0.729. The most promising combinations of test proteins were FGF-23, TPO, and IL-13, reaching a sensitivity of 0.7 and a specificity of 0.7. The combination of FGF-23 and TPO scored highest with sensitivity of 0.7 and specificity of 0.8. Its mean that the combination of FGF-23 and TPO has the highest value for the diagnosis of CRC in our study.
CONCLUSION: A protein biochip composed of proteins found to be elevated in the feces of colorectal cancer patients has great potential as a noninvasive diagnostic for colorectal cancer. The addition of new protein biomarkers and technologies, as they are discovered, is an excellent avenue of future research. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.
推荐引用方式
GB/T 7714：

Wang Hui-Peng,Wang Yang-Yun,Pan Jie, et al. Evaluation of specific fecal protein biochips for the diagnosis of colorectal cancer [J].WORLD JOURNAL OF GASTROENTEROLOGY,2014,20(5):1332-1339.
APA：

Wang Hui-Peng,Wang Yang-Yun,Pan Jie,Cen Rong,&Cai Yuan-Kun.(2014).Evaluation of specific fecal protein biochips for the diagnosis of colorectal cancer .WORLD JOURNAL OF GASTROENTEROLOGY,20(5):1332-1339.
MLA：

Wang Hui-Peng, et al. "Evaluation of specific fecal protein biochips for the diagnosis of colorectal cancer" .WORLD JOURNAL OF GASTROENTEROLOGY 20,5(2014):1332-1339.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：63  下载次数：0

分享到：

浏览次数：63

下载次数：0

打印次数：0