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Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis 期刊论文

编号：

3e431b99-d2e1-4e4f-9e59-be14de6398d4
作者：

Zhang, XiaoJun#^[1]Leung, Feung Ping^[1];Hsiao, Wendy W. L.^[1];Tan, Shun^[1];Li, Shao^[2,3];Xu, HongXi(徐宏喜)^[4]Sung, Joseph J. Y.^[5];Bian, ZhaoXiang*^[1]
语种：

英文
期刊：

WORLD JOURNAL OF GASTROENTEROLOGY ISSN：1007-9327 2012 年 18 卷 23 期 (2914 - 2928) ; JUN 21
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关键词：

Inflammatory bowel disease Trinitrobenzene sulfonic acid Two-dimensional electrophoresis-based proteomic technique Dorsal root ganglia Spinal cord
摘要：

AIM: To investigate proteomic changes in spinal cord and dorsal root ganglia (DRG) of rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis.
METHODS: The colonic myeloperoxidase (MPO) activity and tumor necrosis factor-alpha (TNF-alpha) level were determined. A two-dimensional electrophoresis (2-DE)-based proteomic technique was used to profile the global protein expression changes in the DRG and spinal cord of the rats with acute colitis induced by intracolonic injection of TNBS.
RESULTS: TNBS group showed significantly elevated colonic MPO activity and increased TNF-alpha level. The proteins derived from lumbosacral enlargement of the spinal cord and DRG were resolved by 2-DE; and 26 and 19 proteins that displayed significantly different expression levels in the DRG and spinal cord were identified respectively. Altered proteins were found to be involved in a number of biological functions, such as inflammation/immunity, cell signaling, redox regulation, sulfate transport and cellular metabolism. The overexpression of the protein similar to potassium channel tetramerisation domain containing protein 12 (Kctd 12) and low expression of proteasome subunit alpha type-1 (psma) were validated by Western blotting analysis.
CONCLUSION: TNBS-induced colitis has a profound impact on protein profiling in the nervous system. This result helps understand the neurological pathogenesis of inflammatory bowel disease. (C) 2012 Baishideng. All rights reserved.
推荐引用方式
GB/T 7714：

Zhang Xiao-Jun,Leung Feung Ping,Hsiao Wendy W. L., et al. Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis [J].WORLD JOURNAL OF GASTROENTEROLOGY,2012,18(23):2914-2928.
APA：

Zhang Xiao-Jun,Leung Feung Ping,Hsiao Wendy W. L.,Tan Shun,&Bian Zhao-Xiang.(2012).Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis .WORLD JOURNAL OF GASTROENTEROLOGY,18(23):2914-2928.
MLA：

Zhang Xiao-Jun, et al. "Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis" .WORLD JOURNAL OF GASTROENTEROLOGY 18,23(2012):2914-2928.
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