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The effects of PTBP3 silencing on the proliferation and differentiation of MKN45 human gastric cancer cells 期刊论文

编号：

3c5bcd1e-8b47-49e5-9b49-6730e3b15aff
作者：

Chen, Bin(陈彬)#^[1]Zhao, AiGuang(赵爱光)*^[1]Shao, Jin^[2,3,4];Mu, XiaoYan^[1];Jiang, Lin^[2,3,4];Liu, JianWen^[2,3,4];
语种：

英文
期刊：

LIFE SCIENCES ISSN：0024-3205 2014 年 114 卷 1 期 (29 - 35) ; SEP 26
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关键词：

PTBP3 Gastric cancer differentiation Proliferation
摘要：

Aims: PTBP3 overexpression inhibits the differentiation of leukemia cells; however, its effects on the differentiation and proliferation of solid cancer cells remain unclear. Thus, the impact of PTBP3 on the differentiation and proliferation of gastric cancer cells was investigated.
Main methods: PTBP3 expression was analyzed in normal and tumor tissues using immunohistochemistry. A xenograft model was established in nude mice by subcutaneous injection of untransfected human gastric cancer MKN45 cells or those expressing a control vector or PTBP3 siRNA. We analyzed the tumor inhibition rate, the expression of PTBP3, the PCNA-positive rate and the serum levels of CEA, CA199, CA125, LDH, ALP and gamma-GT in different groups.
Key findings: The tumor weights in the PTBP3 siRNA group were significantly lower than that of the MKN45 cell control group (P < 0.001). Immunohistochemistry analysis of PCNA expression revealed that it was markedly reduced after PTBP3 silencing. ELISAs showed that the serum levels of CEA and CA199 tumor markers as well as LDH and ALP were reduced after PTBP3 silencing. Transmission electron microscopy revealed that MKN45 cells expressing PTBP3 siRNA had reduced nuclear-to-cytoplasmic ratio and regular nuclei, suggesting differentiation.
Significance: PTBP3 may promote proliferation and inhibit the differentiation of human gastric cancer MKN45 cells. (C) 2014 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Chen Bin,Zhao Ai-Guang,Shao Jin, et al. The effects of PTBP3 silencing on the proliferation and differentiation of MKN45 human gastric cancer cells [J].LIFE SCIENCES,2014,114(1):29-35.
APA：

Chen Bin,Zhao Ai-Guang,Shao Jin,Mu Xiao-Yan,&Liu Jian-Wen.(2014).The effects of PTBP3 silencing on the proliferation and differentiation of MKN45 human gastric cancer cells .LIFE SCIENCES,114(1):29-35.
MLA：

Chen Bin, et al. "The effects of PTBP3 silencing on the proliferation and differentiation of MKN45 human gastric cancer cells" .LIFE SCIENCES 114,1(2014):29-35.
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