Trifolirhizin is a compound isolated from Sophora flavescens. It has been shown to exert cytotoxicity on several cancer cell lines. However, the underlying mechanism remains unknown. MKN45 cells were used as a research model. We assessed the cytotoxicity of trifolirhizin to MKN45 by MTT. Hoechst staining and TUNEL method were used to demonstrate apoptosis. Flow cytometry was used to determine cell cycle and ratio of apoptosis. Caspase activity assay was used to examine the activation of caspase cascade pathways. Western blotting was used to explore the protein levels. Consistently, trifolirhizin inhibited MKN45 xenograft tumor growth in vivo. Trifolirhizin caused a significantly decreased proliferation of MKN45 cells in a time- and dose-dependent manner, with IC50 values of 33.27 +/- 2.06 mu g/ml at 48 h. Western blot assay manifested that trifolirhizin activated the EGFR-MAPK signaling pathways. This study indicated that trifolirhizin may be a therapeutic application in human gastric cancer therapy.
[1]Fudan Univ, Huadong Hosp, Dept Tradit Chinese Med, Shanghai 200040, Peoples R China.
[2]Fudan Univ, Huadong Hosp, Dept Gastroenterol, Shanghai 200040, Peoples R China.
[3]Shanghai Univ Tradit Chinese Med, Baoshan Hosp Integrated Tradit Chinese & Western, Dept Oncol, Shanghai 201999, Peoples R China.
[4]Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Nursing, Ward 19, Shanghai 200032, Peoples R China.
[5]Fudan Univ, Huadong Hosp, Dept Pharm, 221 West YanAn Rd, Shanghai 200040, Peoples R China.
(8.0+极速模式)