首页 / 院系成果 / 成果详情页

Selective In Vivo Targeting of Human Liver Tumors by Optimized AAV3 Vectors in a Murine Xenograft Model 期刊论文

编号：

3a528610-0c92-4e6b-b7a8-260d12942f3a
作者：

Ling, Chen#^[1,2]Wang, Yuan#^[1,2,7,8]Zhang, Yuanhui^[1,2,7,8];Ejjigani, Anila^[7];Yin, Zifei^[1,2,7];Lu, Yuan^[3];Wang, Lina^[1,2,7];Wang, Meng^[7,8];Li, Jun^[7];Hu, Zhongbo^[1];Aslanidi, George V.^[1,2];Zhong, Li^[9];Gao, Guangping^[9];Srivastava, Arun^[1,2,4,5,6];Ling, Changquan(凌昌全)*^[7,8,10]
语种：

英文
期刊：

HUMAN GENE THERAPY ISSN：1043-0342 2014 年 25 卷 12 期 (1023 - 1034) ; DEC 1
收录：
摘要：

Current challenges for recombinant adeno-associated virus (rAAV) vector-based cancer treatment include the low efficiency and the lack of specificity in vivo. rAAV serotype 3 (rAAV3) vectors have previously been shown to be ineffective in normal mouse tissues following systemic administration. In the present study, we report that rAAV3 vectors can efficiently target and transduce various human liver cancer cells in vivo. Elimination of specific surface-exposed serine and threonine residues on rAAV3 capsids results in further augmentation in the transduction efficiency of these vectors, without any change in the viral tropism and cellular receptor interactions. In addition, we have identified a potential chemotherapy drug, shikonin, as a multifunctional compound to inhibit liver tumor growth as well as to significantly enhance the efficacy of rAAV vector-based gene therapy in vivo. Furthermore, we also document that suppression of tumorigenesis in a human liver cancer xenograft model can be achieved through systemic administration of the optimized rAAV3 vectors carrying a therapeutic gene, and shikonin at a dose that does not lead to liver damage. Our research provides a novel means to achieve not only targeted delivery but also the potential for gene therapy of human liver cancer.
推荐引用方式
GB/T 7714：

Ling Chen,Wang Yuan,Zhang Yuanhui, et al. Selective In Vivo Targeting of Human Liver Tumors by Optimized AAV3 Vectors in a Murine Xenograft Model [J].HUMAN GENE THERAPY,2014,25(12):1023-1034.
APA：

Ling Chen,Wang Yuan,Zhang Yuanhui,Ejjigani Anila,&Ling Changquan.(2014).Selective In Vivo Targeting of Human Liver Tumors by Optimized AAV3 Vectors in a Murine Xenograft Model .HUMAN GENE THERAPY,25(12):1023-1034.
MLA：

Ling Chen, et al. "Selective In Vivo Targeting of Human Liver Tumors by Optimized AAV3 Vectors in a Murine Xenograft Model" .HUMAN GENE THERAPY 25,12(2014):1023-1034.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：4  下载次数：0

分享到：

浏览次数：4

下载次数：0

打印次数：0