Objective. This study aimed to analyze the differential metabolites and their metabolic pathways from the serum of patients with hepatitis B cirrhosis, with two typical patterns of Gan Dan Shi Re (GDSR) and Gan Shen Yin Xu (GSYX) based on the theory of traditional Chinese medicine (TCM). It also investigated the variation in the internal material basis for the two types of patterns and provided an objective basis for classifying TCM patterns using metabolomic techniques. Methods. The serum samples taken from 111 qualified patients (40 GDSR cases, 41 GSYX cases, and 30 Latent Pattern (LP) cases with no obvious pattern characters) and 60 healthy volunteers were tested to identify the differential substances relevant to hepatitis B cirrhosis and the two typical TCM patterns under the gas chromatography-time-of-flight mass spectrometry platform. The relevant metabolic pathways of differential substances were analyzed using multidimensional statistical analysis. Results. After excluding the influence of LP groups, six common substances were found in GDSR and GSYX patterns, which were mainly involved in the metabolic pathways of glycine, serine, threonine, and phenylalanine. Eight specific metabolites involved in the metabolic pathways of linoleic, glycine, threonine, and serine existed in the two patterns. Conclusions. The data points on the metabolic spectrum were found to be well distributed among the differential substances between the two typical TCM patterns of patients with hepatitis B cirrhosis using metabolomic techniques. The differential expression of these substances between GDSR and GSYX patterns provided an important objective basis for the scientific nature of TCM pattern classification at the metabolic level.
[1]Shanghai Univ Tradit Chinese Med, Key Lab Liver & Kidney Dis, Key Lab Clin Chinese Med, Inst Liver Dis,Shuguang Hosp,Minist Educ, 258 Zhangheng Rd, Shanghai 201203, Peoples R China.
[2]Fujian Univ Tradit Chinese Med, Xiamen Hosp Tradit Chinese Med, Fuzhou 361000, Fujian, Peoples R China.
[3]Shanghai Univ Tradit Chinese Med, Shanghai Municipal Educ Commiss, E Inst Tradit Chinese Internal Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China.
[4]Baoshan Dist Hosp Integrated Tradit Chinese & Wes, Cent Lab, Shanghai 201999, Peoples R China.
(8.0+极速模式)