Macroautophagy/autophagy is a cellular process in which cytosolic contents are degraded by lysosome in response to various stress conditions. Apart from its role in the maintenance of cellular homeostasis, autophagy also involves in regulation of cell cycle progression under nutrient-deprivation conditions. However, whether and how autophagy is regulated by the cell cycle especially during mitosis remains largely undefined. Here we show that WIPI2/ATG18B (WD repeat domain, phosphoinositide interacting 2), an autophagy-related (ATG) protein that plays a critical role in autophagosome biogenesis, is a direct substrate of CUL4-RING ubiquitin ligases (CRL4s). Upon mitosis induction, CRL4s are activated via neddylation, and recruit WIPI2 via DDB1 (damage specific DNA binding protein 1), leading to polyubiquitination and proteasomal degradation of WIPI2 and suppression of autophagy. The WIPI2 protein level and autophagy during mitosis could be rescued by knockdown of CRL4s or treatment with MLN4924/Pevonedistat, a selective inhibitor of CRLs, via suppression of NAE1 (NEDD8 activating enzyme E1 subunit 1). Moreover, restoration of WIPI2 rescues autophagy during mitosis and leads to mitotic slippage and cell senescence. Our study thus discovers a novel function of CRL4s in autophagy by targeting WIPI2 for polyubiquitination and proteasomal degradation during mitosis.
[1]Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore, Singapore.
[2]Francis Crick Inst, Mol Cell Biol Autophagy Lab, London, England.
[3]Acad Sinica, Inst Biol Chem, Taipei, Taiwan.
[4]Zhejiang Univ, Sch Publ Hlth, Hangzhou, Zhejiang, Peoples R China.
[5]Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai, Peoples R China.
[6]Zhejiang Univ, Dept Biochem & Mol Biol, Sch Med, Hangzhou, Zhejiang, Peoples R China.
[7]Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore, Singapore.
[8]Univ Toronto, Fac Arts & Sci, Life Sci Program, Toronto, ON, Canada.
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